ssr_primer
Primer Details for TRINITY_DN73735_c1_g5_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCAACAGAAAGTGCACCCA | TACTTGGCGCAATGAGTCGT | Start: 569 |
| Tm | 60.034 | 60.038 | End: 677 |
| Size | 20 | 20 | 109 |
| Primer 2 details |
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| Sequence | GGTGGCATTAGAGGCGTCTT | TGGGTGCACTTTCTGTTGGT | Start: 434 |
| Tm | 60.108 | 60.034 | End: 588 |
| Size | 20 | 20 | 155 |
| Primer 3 details |
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| Sequence | TCATGTGAATCACCACCGCA | GGACTTCACACCACACCACA | Start: 32 |
| Tm | 59.964 | 60.107 | End: 272 |
| Size | 20 | 20 | 241 |
| Primer 4 details |
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| Sequence | TCATGTGAATCACCACCGCA | TGGACTTCACACCACACCAC | Start: 32 |
| Tm | 59.964 | 60.107 | End: 273 |
| Size | 20 | 20 | 242 |
| Primer 5 details |
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| Sequence | GGTGGCATTAGAGGCGTCTT | AATGAGTCGTTCCCTGCAGG | Start: 434 |
| Tm | 60.108 | 60.036 | End: 667 |
| Size | 20 | 20 | 234 |
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