ssr_primer
Primer Details for TRINITY_DN73731_c5_g4_i4 |
|---|
| (A)13taaaaaat(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGAGTCATCAAGATCCCCG | GCGACGCGGGTCTCTTATTA | Start: 310 |
| Tm | 59.967 | 59.97 | End: 462 |
| Size | 20 | 20 | 153 |
| Primer 2 details |
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| Sequence | TAATAAGAGACCCGCGTCGC | TTTTCCCCCGTGTCTATGGC | Start: 443 |
| Tm | 59.97 | 60.035 | End: 665 |
| Size | 20 | 20 | 223 |
| Primer 3 details |
|---|
| Sequence | TAATAAGAGACCCGCGTCGC | TGGTGCGAGATGAAATGCCT | Start: 443 |
| Tm | 59.97 | 60.035 | End: 615 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | TAATAAGAGACCCGCGTCGC | CCCCGTGTCTATGGCTTTGT | Start: 443 |
| Tm | 59.97 | 60.035 | End: 660 |
| Size | 20 | 20 | 218 |
| Primer 5 details |
|---|
| Sequence | GCCATAGACACGGGGGAAAA | GCGCCAGGTAGGAAGAAACT | Start: 646 |
| Tm | 60.035 | 60.036 | End: 919 |
| Size | 20 | 20 | 274 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGAGTCATCAAGATCCCCG | GCGACGCGGGTCTCTTATTA | Start: 310 |
| Tm | 59.967 | 59.97 | End: 462 |
| Size | 20 | 20 | 153 |
| Primer 2 details |
|---|
| Sequence | TAATAAGAGACCCGCGTCGC | TTTTCCCCCGTGTCTATGGC | Start: 443 |
| Tm | 59.97 | 60.035 | End: 665 |
| Size | 20 | 20 | 223 |
| Primer 3 details |
|---|
| Sequence | TAATAAGAGACCCGCGTCGC | TGGTGCGAGATGAAATGCCT | Start: 443 |
| Tm | 59.97 | 60.035 | End: 615 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | TAATAAGAGACCCGCGTCGC | CCCCGTGTCTATGGCTTTGT | Start: 443 |
| Tm | 59.97 | 60.035 | End: 660 |
| Size | 20 | 20 | 218 |
| Primer 5 details |
|---|
| Sequence | GCCATAGACACGGGGGAAAA | GCGCCAGGTAGGAAGAAACT | Start: 646 |
| Tm | 60.035 | 60.036 | End: 919 |
| Size | 20 | 20 | 274 |
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