ssr_primer
Primer Details for TRINITY_DN73689_c3_g1_i6 |
|---|
| (T)22: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGGAGAGTGAGCAATGGC | CGGTGTTTCAGGTACGCTCT | Start: 98 |
| Tm | 60.107 | 60.039 | End: 207 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | AGAGCGTACCTGAAACACCG | TAATGGCTCTAACGGCGTGG | Start: 188 |
| Tm | 60.039 | 60.179 | End: 390 |
| Size | 20 | 20 | 203 |
| Primer 3 details |
|---|
| Sequence | ATGGGAGAGTGAGCAATGGC | TTTATCGCAGGGGTAGGTGC | Start: 98 |
| Tm | 60.107 | 59.821 | End: 254 |
| Size | 20 | 20 | 157 |
| Primer 4 details |
|---|
| Sequence | AAGTCGCAAGTGCTCCAGTT | TGACTTTAGCTGGCAGCACA | Start: 254 |
| Tm | 60.179 | 59.892 | End: 472 |
| Size | 20 | 20 | 219 |
| Primer 5 details |
|---|
| Sequence | AAAGTCGCAAGTGCTCCAGT | TGACTTTAGCTGGCAGCACA | Start: 253 |
| Tm | 60.179 | 59.892 | End: 472 |
| Size | 20 | 20 | 220 |
(T)28: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGGAGAGTGAGCAATGGC | CGGTGTTTCAGGTACGCTCT | Start: 98 |
| Tm | 60.107 | 60.039 | End: 207 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | AGAGCGTACCTGAAACACCG | TAATGGCTCTAACGGCGTGG | Start: 188 |
| Tm | 60.039 | 60.179 | End: 390 |
| Size | 20 | 20 | 203 |
| Primer 3 details |
|---|
| Sequence | ATGGGAGAGTGAGCAATGGC | TTTATCGCAGGGGTAGGTGC | Start: 98 |
| Tm | 60.107 | 59.821 | End: 254 |
| Size | 20 | 20 | 157 |
| Primer 4 details |
|---|
| Sequence | AAGTCGCAAGTGCTCCAGTT | TGACTTTAGCTGGCAGCACA | Start: 254 |
| Tm | 60.179 | 59.892 | End: 472 |
| Size | 20 | 20 | 219 |
| Primer 5 details |
|---|
| Sequence | AAAGTCGCAAGTGCTCCAGT | TGACTTTAGCTGGCAGCACA | Start: 253 |
| Tm | 60.179 | 59.892 | End: 472 |
| Size | 20 | 20 | 220 |
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