ssr_primer
Primer Details for TRINITY_DN73684_c3_g1_i8 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCACAGCATGACATGGGCAT | GCGGTGCTTTGTGTGCTTTA | Start: 1354 |
| Tm | 60.034 | 59.97 | End: 1539 |
| Size | 20 | 20 | 186 |
| Primer 2 details |
|---|
| Sequence | GCAAAACCGTGCCTGTTCAA | GCGGTGCTTTGTGTGCTTTA | Start: 1268 |
| Tm | 60.179 | 59.97 | End: 1539 |
| Size | 20 | 20 | 272 |
| Primer 3 details |
|---|
| Sequence | GCAAAACCGTGCCTGTTCAA | ATGCCCATGTCATGCTGTGA | Start: 1268 |
| Tm | 60.179 | 60.034 | End: 1373 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | TCACAGCATGACATGGGCAT | GTTATCGGCGGTGCTTTGTG | Start: 1354 |
| Tm | 60.034 | 60.179 | End: 1546 |
| Size | 20 | 20 | 193 |
| Primer 5 details |
|---|
| Sequence | CACTGTGAACGACAATGCCG | GTGCGGTCACGAAAATCACC | Start: 549 |
| Tm | 60.111 | 60.11 | End: 726 |
| Size | 20 | 20 | 178 |
(T)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCACAGCATGACATGGGCAT | GCGGTGCTTTGTGTGCTTTA | Start: 1354 |
| Tm | 60.034 | 59.97 | End: 1539 |
| Size | 20 | 20 | 186 |
| Primer 2 details |
|---|
| Sequence | GCAAAACCGTGCCTGTTCAA | GCGGTGCTTTGTGTGCTTTA | Start: 1268 |
| Tm | 60.179 | 59.97 | End: 1539 |
| Size | 20 | 20 | 272 |
| Primer 3 details |
|---|
| Sequence | GCAAAACCGTGCCTGTTCAA | ATGCCCATGTCATGCTGTGA | Start: 1268 |
| Tm | 60.179 | 60.034 | End: 1373 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | TCACAGCATGACATGGGCAT | GTTATCGGCGGTGCTTTGTG | Start: 1354 |
| Tm | 60.034 | 60.179 | End: 1546 |
| Size | 20 | 20 | 193 |
| Primer 5 details |
|---|
| Sequence | CACTGTGAACGACAATGCCG | GTGCGGTCACGAAAATCACC | Start: 549 |
| Tm | 60.111 | 60.11 | End: 726 |
| Size | 20 | 20 | 178 |
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