ssr_primer
Primer Details for TRINITY_DN73683_c1_g1_i3 |
|---|
| (A)24: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAAACATGTCGCGGTCAGA | CTCACCTGGCCATTCGAGTT | Start: 235 |
| Tm | 59.967 | 60.036 | End: 361 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | CCTGAAGAAGCGCCTTGAGA | TCTGACCGCGACATGTTTCA | Start: 48 |
| Tm | 60.037 | 59.967 | End: 254 |
| Size | 20 | 20 | 207 |
| Primer 3 details |
|---|
| Sequence | TCCTGAAGAAGCGCCTTGAG | TCTGACCGCGACATGTTTCA | Start: 47 |
| Tm | 60.037 | 59.967 | End: 254 |
| Size | 20 | 20 | 208 |
| Primer 4 details |
|---|
| Sequence | TGAAACATGTCGCGGTCAGA | TGTTGGCATTGCACTTACGC | Start: 235 |
| Tm | 59.967 | 60.04 | End: 479 |
| Size | 20 | 20 | 245 |
| Primer 5 details |
|---|
| Sequence | AACTCGAATGGCCAGGTGAG | TGTTGGCATTGCACTTACGC | Start: 342 |
| Tm | 60.036 | 60.04 | End: 479 |
| Size | 20 | 20 | 138 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAAACATGTCGCGGTCAGA | CTCACCTGGCCATTCGAGTT | Start: 235 |
| Tm | 59.967 | 60.036 | End: 361 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | CCTGAAGAAGCGCCTTGAGA | TCTGACCGCGACATGTTTCA | Start: 48 |
| Tm | 60.037 | 59.967 | End: 254 |
| Size | 20 | 20 | 207 |
| Primer 3 details |
|---|
| Sequence | TCCTGAAGAAGCGCCTTGAG | TCTGACCGCGACATGTTTCA | Start: 47 |
| Tm | 60.037 | 59.967 | End: 254 |
| Size | 20 | 20 | 208 |
| Primer 4 details |
|---|
| Sequence | TGAAACATGTCGCGGTCAGA | TGTTGGCATTGCACTTACGC | Start: 235 |
| Tm | 59.967 | 60.04 | End: 479 |
| Size | 20 | 20 | 245 |
| Primer 5 details |
|---|
| Sequence | AACTCGAATGGCCAGGTGAG | TGTTGGCATTGCACTTACGC | Start: 342 |
| Tm | 60.036 | 60.04 | End: 479 |
| Size | 20 | 20 | 138 |
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