ssr_primer
Primer Details for TRINITY_DN73448_c5_g3_i2 |
|---|
| (A)20: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCTAACTTCGCTGTCGCTC | TTGGATCCGACCGAAACCAG | Start: 97 |
| Tm | 60.109 | 60.037 | End: 324 |
| Size | 20 | 20 | 228 |
| Primer 2 details |
|---|
| Sequence | ACCTAACTTCGCTGTCGCTC | CACCAGGCCCCAAAACTTTG | Start: 97 |
| Tm | 60.109 | 59.893 | End: 341 |
| Size | 20 | 20 | 245 |
| Primer 3 details |
|---|
| Sequence | GCACTCGTTATGCTCCGACT | TTGGATCCGACCGAAACCAG | Start: 149 |
| Tm | 60.179 | 60.037 | End: 324 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
|---|
| Sequence | AAACCAGAATCGGCCCAACT | CACCAGGCCCCAAAACTTTG | Start: 226 |
| Tm | 59.888 | 59.893 | End: 341 |
| Size | 20 | 20 | 116 |
| Primer 5 details |
|---|
| Sequence | ACCTAACTTCGCTGTCGCTC | AGTTGGGCCGATTCTGGTTT | Start: 97 |
| Tm | 60.109 | 59.888 | End: 245 |
| Size | 20 | 20 | 149 |
(A)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCTAACTTCGCTGTCGCTC | TTGGATCCGACCGAAACCAG | Start: 97 |
| Tm | 60.109 | 60.037 | End: 324 |
| Size | 20 | 20 | 228 |
| Primer 2 details |
|---|
| Sequence | ACCTAACTTCGCTGTCGCTC | CACCAGGCCCCAAAACTTTG | Start: 97 |
| Tm | 60.109 | 59.893 | End: 341 |
| Size | 20 | 20 | 245 |
| Primer 3 details |
|---|
| Sequence | GCACTCGTTATGCTCCGACT | TTGGATCCGACCGAAACCAG | Start: 149 |
| Tm | 60.179 | 60.037 | End: 324 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
|---|
| Sequence | AAACCAGAATCGGCCCAACT | CACCAGGCCCCAAAACTTTG | Start: 226 |
| Tm | 59.888 | 59.893 | End: 341 |
| Size | 20 | 20 | 116 |
| Primer 5 details |
|---|
| Sequence | ACCTAACTTCGCTGTCGCTC | AGTTGGGCCGATTCTGGTTT | Start: 97 |
| Tm | 60.109 | 59.888 | End: 245 |
| Size | 20 | 20 | 149 |
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