ssr_primer
Primer Details for TRINITY_DN73408_c2_g2_i2 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTTGCAGTATACGGTGGCC | CGGATCGTTTTTCGTCGCTC | Start: 645 |
| Tm | 60.108 | 59.977 | End: 748 |
| Size | 20 | 20 | 104 |
| Primer 2 details |
|---|
| Sequence | AGTTGCAGTATACGGTGGCC | GGGAGGTCCGGATCGTTTTT | Start: 645 |
| Tm | 60.108 | 60.036 | End: 756 |
| Size | 20 | 20 | 112 |
| Primer 3 details |
|---|
| Sequence | ACAGCTGATGTACCGTCACC | GTTCCCCTTCCCCTGTAACG | Start: 230 |
| Tm | 59.753 | 60.036 | End: 368 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | GAGCGACGAAAAACGATCCG | TGTTGCAACGTTGGAGCATC | Start: 729 |
| Tm | 59.977 | 59.689 | End: 891 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
|---|
| Sequence | AAAAACGATCCGGACCTCCC | TGTTGCAACGTTGGAGCATC | Start: 737 |
| Tm | 60.036 | 59.689 | End: 891 |
| Size | 20 | 20 | 155 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTTGCAGTATACGGTGGCC | CGGATCGTTTTTCGTCGCTC | Start: 645 |
| Tm | 60.108 | 59.977 | End: 748 |
| Size | 20 | 20 | 104 |
| Primer 2 details |
|---|
| Sequence | AGTTGCAGTATACGGTGGCC | GGGAGGTCCGGATCGTTTTT | Start: 645 |
| Tm | 60.108 | 60.036 | End: 756 |
| Size | 20 | 20 | 112 |
| Primer 3 details |
|---|
| Sequence | ACAGCTGATGTACCGTCACC | GTTCCCCTTCCCCTGTAACG | Start: 230 |
| Tm | 59.753 | 60.036 | End: 368 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | GAGCGACGAAAAACGATCCG | TGTTGCAACGTTGGAGCATC | Start: 729 |
| Tm | 59.977 | 59.689 | End: 891 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
|---|
| Sequence | AAAAACGATCCGGACCTCCC | TGTTGCAACGTTGGAGCATC | Start: 737 |
| Tm | 60.036 | 59.689 | End: 891 |
| Size | 20 | 20 | 155 |
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