ssr_primer
Primer Details for TRINITY_DN73338_c4_g3_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGAGTTGGACGTTTTGCGC | TCCGTCTGCTCTAGCTCACT | Start: 784 |
| Tm | 59.971 | 60.035 | End: 1013 |
| Size | 20 | 20 | 230 |
| Primer 2 details |
|---|
| Sequence | CGCCCTTCTTTCTCCCAACT | GCGCAAAACGTCCAACTCTT | Start: 641 |
| Tm | 59.963 | 59.971 | End: 803 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | CCCCCAGTCCACCATTAACC | AGTTGGGAGAAAGAAGGGCG | Start: 502 |
| Tm | 60.034 | 59.963 | End: 660 |
| Size | 20 | 20 | 159 |
| Primer 4 details |
|---|
| Sequence | GTTGGACGTTTTGCGCTGAT | TCCGTCTGCTCTAGCTCACT | Start: 788 |
| Tm | 60.04 | 60.035 | End: 1013 |
| Size | 20 | 20 | 226 |
| Primer 5 details |
|---|
| Sequence | CGCCCTTCTTTCTCCCAACT | ATCAGCGCAAAACGTCCAAC | Start: 641 |
| Tm | 59.963 | 60.04 | End: 807 |
| Size | 20 | 20 | 167 |
(T)11cgtttaaatacgtttggtgccttacggcaggtccagcggtgtcctttttcaacccatcaggctgcattctctttacgcgaccatcgaaaattt(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGAGTTGGACGTTTTGCGC | TCCGTCTGCTCTAGCTCACT | Start: 784 |
| Tm | 59.971 | 60.035 | End: 1013 |
| Size | 20 | 20 | 230 |
| Primer 2 details |
|---|
| Sequence | CGCCCTTCTTTCTCCCAACT | GCGCAAAACGTCCAACTCTT | Start: 641 |
| Tm | 59.963 | 59.971 | End: 803 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | CCCCCAGTCCACCATTAACC | AGTTGGGAGAAAGAAGGGCG | Start: 502 |
| Tm | 60.034 | 59.963 | End: 660 |
| Size | 20 | 20 | 159 |
| Primer 4 details |
|---|
| Sequence | GTTGGACGTTTTGCGCTGAT | TCCGTCTGCTCTAGCTCACT | Start: 788 |
| Tm | 60.04 | 60.035 | End: 1013 |
| Size | 20 | 20 | 226 |
| Primer 5 details |
|---|
| Sequence | CGCCCTTCTTTCTCCCAACT | ATCAGCGCAAAACGTCCAAC | Start: 641 |
| Tm | 59.963 | 60.04 | End: 807 |
| Size | 20 | 20 | 167 |
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