ssr_primer
Primer Details for TRINITY_DN73310_c0_g1_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACAGAGGCGCTATGATGCA | TTGTGACTTTCACCTCGGGG | Start: 843 |
| Tm | 59.821 | 59.892 | End: 1054 |
| Size | 20 | 20 | 212 |
| Primer 2 details |
|---|
| Sequence | AGCGCACACTAGCTGATCAA | TTGTGACTTTCACCTCGGGG | Start: 821 |
| Tm | 59.753 | 59.892 | End: 1054 |
| Size | 20 | 20 | 234 |
| Primer 3 details |
|---|
| Sequence | CAGAGGCGCTATGATGCAGT | TTGTGACTTTCACCTCGGGG | Start: 845 |
| Tm | 60.25 | 59.892 | End: 1054 |
| Size | 20 | 20 | 210 |
| Primer 4 details |
|---|
| Sequence | ACAGAGGCGCTATGATGCAG | TTGTGACTTTCACCTCGGGG | Start: 844 |
| Tm | 60.25 | 59.892 | End: 1054 |
| Size | 20 | 20 | 211 |
| Primer 5 details |
|---|
| Sequence | AACAGAGGCGCTATGATGCA | GGGCCCTCCAATTCTGTAGG | Start: 843 |
| Tm | 59.821 | 59.816 | End: 1036 |
| Size | 20 | 20 | 194 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACAGAGGCGCTATGATGCA | TTGTGACTTTCACCTCGGGG | Start: 843 |
| Tm | 59.821 | 59.892 | End: 1054 |
| Size | 20 | 20 | 212 |
| Primer 2 details |
|---|
| Sequence | AGCGCACACTAGCTGATCAA | TTGTGACTTTCACCTCGGGG | Start: 821 |
| Tm | 59.753 | 59.892 | End: 1054 |
| Size | 20 | 20 | 234 |
| Primer 3 details |
|---|
| Sequence | CAGAGGCGCTATGATGCAGT | TTGTGACTTTCACCTCGGGG | Start: 845 |
| Tm | 60.25 | 59.892 | End: 1054 |
| Size | 20 | 20 | 210 |
| Primer 4 details |
|---|
| Sequence | ACAGAGGCGCTATGATGCAG | TTGTGACTTTCACCTCGGGG | Start: 844 |
| Tm | 60.25 | 59.892 | End: 1054 |
| Size | 20 | 20 | 211 |
| Primer 5 details |
|---|
| Sequence | AACAGAGGCGCTATGATGCA | GGGCCCTCCAATTCTGTAGG | Start: 843 |
| Tm | 59.821 | 59.816 | End: 1036 |
| Size | 20 | 20 | 194 |
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