ssr_primer
Primer Details for TRINITY_DN73295_c3_g1_i1 |
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| (T)19: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGGTTTGTAATTGGCGGGA | TCGTTTTTGGAGGTGTTTGTCG | Start: 36 |
| Tm | 60.961 | 59.904 | End: 193 |
| Size | 20 | 22 | 158 |
| Primer 2 details |
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| Sequence | CCGGTTTGTAATTGGCGGGA | TGGAGGTGTTTGTCGGAAATCA | Start: 36 |
| Tm | 60.961 | 60.159 | End: 186 |
| Size | 20 | 22 | 151 |
| Primer 3 details |
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| Sequence | CGGTTTGTAATTGGCGGGAT | TCGTTTTTGGAGGTGTTTGTCG | Start: 37 |
| Tm | 58.9 | 59.904 | End: 193 |
| Size | 20 | 22 | 157 |
| Primer 4 details |
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| Sequence | CGGTTTGTAATTGGCGGGAT | TGGAGGTGTTTGTCGGAAATCA | Start: 37 |
| Tm | 58.9 | 60.159 | End: 186 |
| Size | 20 | 22 | 150 |
| Primer 5 details |
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| Sequence | CCGGTTTGTAATTGGCGGGA | AGGTACTTAATCGCGCACAA | Start: 36 |
| Tm | 60.961 | 57.626 | End: 151 |
| Size | 20 | 20 | 116 |
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