ssr_primer
Primer Details for TRINITY_DN73288_c5_g2_i3 |
|---|
| (T)25: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGAATGCGAACTGGGTGT | GTCCAGTCGAGCAAACAGGA | Start: 1911 |
| Tm | 59.891 | 59.966 | End: 2137 |
| Size | 20 | 20 | 227 |
| Primer 2 details |
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| Sequence | TGCATCTTCACGTAGGCCAG | GAGCTGCCCTGTGGAAAGAT | Start: 3413 |
| Tm | 60.108 | 60.035 | End: 3519 |
| Size | 20 | 20 | 107 |
| Primer 3 details |
|---|
| Sequence | CGGCAAGTCGACTGTGTAGT | CGGCCCCTTTGGTAGGTAAG | Start: 691 |
| Tm | 60.039 | 60.107 | End: 798 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | CATCTTCACGTAGGCCAGCT | GATGTAGACGCGGTAGGGTG | Start: 3343 |
| Tm | 59.823 | 59.97 | End: 3454 |
| Size | 20 | 20 | 112 |
| Primer 5 details |
|---|
| Sequence | CATCTTCACGTAGGCCAGCT | GAGCTGCCCTGTGGAAAGAT | Start: 3415 |
| Tm | 59.823 | 60.035 | End: 3519 |
| Size | 20 | 20 | 105 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGAATGCGAACTGGGTGT | GTCCAGTCGAGCAAACAGGA | Start: 1911 |
| Tm | 59.891 | 59.966 | End: 2137 |
| Size | 20 | 20 | 227 |
| Primer 2 details |
|---|
| Sequence | TGCATCTTCACGTAGGCCAG | GAGCTGCCCTGTGGAAAGAT | Start: 3413 |
| Tm | 60.108 | 60.035 | End: 3519 |
| Size | 20 | 20 | 107 |
| Primer 3 details |
|---|
| Sequence | CGGCAAGTCGACTGTGTAGT | CGGCCCCTTTGGTAGGTAAG | Start: 691 |
| Tm | 60.039 | 60.107 | End: 798 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | CATCTTCACGTAGGCCAGCT | GATGTAGACGCGGTAGGGTG | Start: 3343 |
| Tm | 59.823 | 59.97 | End: 3454 |
| Size | 20 | 20 | 112 |
| Primer 5 details |
|---|
| Sequence | CATCTTCACGTAGGCCAGCT | GAGCTGCCCTGTGGAAAGAT | Start: 3415 |
| Tm | 59.823 | 60.035 | End: 3519 |
| Size | 20 | 20 | 105 |
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