ssr_primer
Primer Details for TRINITY_DN73255_c4_g2_i8 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACTGTGTGATGGCATCGGT | GGCAAAACGCAGTGAACCAT | Start: 1300 |
| Tm | 59.963 | 59.968 | End: 1445 |
| Size | 20 | 20 | 146 |
| Primer 2 details |
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| Sequence | ATGGTTCACTGCGTTTTGCC | TGGGTCTTTTTCCAGCAGCA | Start: 1426 |
| Tm | 59.968 | 60.106 | End: 1584 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
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| Sequence | ATGGTTCACTGCGTTTTGCC | CCCAGCACATCTTCCTCTGG | Start: 1426 |
| Tm | 59.968 | 60.107 | End: 1667 |
| Size | 20 | 20 | 242 |
| Primer 4 details |
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| Sequence | ATGGTTCACTGCGTTTTGCC | CCCCAGCACATCTTCCTCTG | Start: 1426 |
| Tm | 59.968 | 60.107 | End: 1668 |
| Size | 20 | 20 | 243 |
| Primer 5 details |
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| Sequence | TCCGTCAAAGATCAGTCGGC | GGCAAAACGCAGTGAACCAT | Start: 1260 |
| Tm | 60.109 | 59.968 | End: 1445 |
| Size | 20 | 20 | 186 |
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