Primer Details for TRINITY_DN73254_c7_g1_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAAATACATTATTCAGTAGGATGGA | TGAGATCAAAAACACTCCCTGA | Start: 4 |
| Tm | 58.056 | 57.295 | End: 175 |
| Size | 27 | 22 | 172 |
| Primer 2 details |
|---|
| Sequence | TGGAAATACATTATTCAGTAGGATGGA | TGAGATCAAAAACACTCCCTGAT | Start: 4 |
| Tm | 58.056 | 57.753 | End: 175 |
| Size | 27 | 23 | 172 |
| Primer 3 details |
|---|
| Sequence | TGGAAATACATTATTCAGTAGGATGGA | ATGAGATCAAAAACACTCCCTGA | Start: 4 |
| Tm | 58.056 | 57.753 | End: 176 |
| Size | 27 | 23 | 173 |
| Primer 4 details |
|---|
| Sequence | ATGGAAATACATTATTCAGTAGGATGG | TGAGATCAAAAACACTCCCTGA | Start: 3 |
| Tm | 57.255 | 57.295 | End: 175 |
| Size | 27 | 22 | 173 |
| Primer 5 details |
|---|
| Sequence | GGAAATACATTATTCAGTAGGATGGAA | TGAGATCAAAAACACTCCCTGA | Start: 5 |
| Tm | 57.207 | 57.295 | End: 175 |
| Size | 27 | 22 | 171 |
