ssr_primer
Primer Details for TRINITY_DN73181_c7_g1_i3 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCGCAGGGAAAACAAAGCG | TGCCGGATTCTCCTACTGGA | Start: 586 |
| Tm | 59.97 | 60.033 | End: 765 |
| Size | 20 | 20 | 180 |
| Primer 2 details |
|---|
| Sequence | AGTCAGCAGCGTTACGAACA | AGGTAGATGACGGTGGGTGA | Start: 2089 |
| Tm | 59.968 | 59.959 | End: 2283 |
| Size | 20 | 20 | 195 |
| Primer 3 details |
|---|
| Sequence | AAATGGCACTGGGTCCACAA | TGCCGGATTCTCCTACTGGA | Start: 563 |
| Tm | 60.106 | 60.033 | End: 765 |
| Size | 20 | 20 | 203 |
| Primer 4 details |
|---|
| Sequence | AAAATGGCACTGGGTCCACA | TGCCGGATTCTCCTACTGGA | Start: 562 |
| Tm | 60.106 | 60.033 | End: 765 |
| Size | 20 | 20 | 204 |
| Primer 5 details |
|---|
| Sequence | GCCGAACTTAGACCCCTGTC | TGTTCGTAACGCTGCTGACT | Start: 1864 |
| Tm | 60.108 | 59.968 | End: 2108 |
| Size | 20 | 20 | 245 |
(T)11gtttttttcttgttgaaattcctgaaaaattgtacccct(C)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCGCAGGGAAAACAAAGCG | TGCCGGATTCTCCTACTGGA | Start: 586 |
| Tm | 59.97 | 60.033 | End: 765 |
| Size | 20 | 20 | 180 |
| Primer 2 details |
|---|
| Sequence | AGTCAGCAGCGTTACGAACA | AGGTAGATGACGGTGGGTGA | Start: 2089 |
| Tm | 59.968 | 59.959 | End: 2283 |
| Size | 20 | 20 | 195 |
| Primer 3 details |
|---|
| Sequence | AAATGGCACTGGGTCCACAA | TGCCGGATTCTCCTACTGGA | Start: 563 |
| Tm | 60.106 | 60.033 | End: 765 |
| Size | 20 | 20 | 203 |
| Primer 4 details |
|---|
| Sequence | AAAATGGCACTGGGTCCACA | TGCCGGATTCTCCTACTGGA | Start: 562 |
| Tm | 60.106 | 60.033 | End: 765 |
| Size | 20 | 20 | 204 |
| Primer 5 details |
|---|
| Sequence | GCCGAACTTAGACCCCTGTC | TGTTCGTAACGCTGCTGACT | Start: 1864 |
| Tm | 60.108 | 59.968 | End: 2108 |
| Size | 20 | 20 | 245 |
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