ssr_primer
Primer Details for TRINITY_DN73174_c5_g1_i4 |
|---|
| (A)47: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTACATGACCGTCACACCA | TCGAAACGTCCCGAGTTGTT | Start: 27 |
| Tm | 60.038 | 59.897 | End: 166 |
| Size | 20 | 20 | 140 |
| Primer 2 details |
|---|
| Sequence | TCCCGTAAAGCGAAGTGACC | GATGTCAAAGGGTCCGAGGG | Start: 552 |
| Tm | 60.038 | 60.108 | End: 741 |
| Size | 20 | 20 | 190 |
| Primer 3 details |
|---|
| Sequence | TCCCGTAAAGCGAAGTGACC | GGATGTCAAAGGGTCCGAGG | Start: 552 |
| Tm | 60.038 | 60.108 | End: 742 |
| Size | 20 | 20 | 191 |
| Primer 4 details |
|---|
| Sequence | TCCCGTAAAGCGAAGTGACC | GGGATGTCAAAGGGTCCGAG | Start: 552 |
| Tm | 60.038 | 60.108 | End: 743 |
| Size | 20 | 20 | 192 |
| Primer 5 details |
|---|
| Sequence | GCTACATGACCGTCACACCA | GAACAACTCGGGGCATTTCG | Start: 27 |
| Tm | 60.038 | 59.832 | End: 241 |
| Size | 20 | 20 | 215 |
(A)22: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTACATGACCGTCACACCA | TCGAAACGTCCCGAGTTGTT | Start: 27 |
| Tm | 60.038 | 59.897 | End: 166 |
| Size | 20 | 20 | 140 |
| Primer 2 details |
|---|
| Sequence | TCCCGTAAAGCGAAGTGACC | GATGTCAAAGGGTCCGAGGG | Start: 552 |
| Tm | 60.038 | 60.108 | End: 741 |
| Size | 20 | 20 | 190 |
| Primer 3 details |
|---|
| Sequence | TCCCGTAAAGCGAAGTGACC | GGATGTCAAAGGGTCCGAGG | Start: 552 |
| Tm | 60.038 | 60.108 | End: 742 |
| Size | 20 | 20 | 191 |
| Primer 4 details |
|---|
| Sequence | TCCCGTAAAGCGAAGTGACC | GGGATGTCAAAGGGTCCGAG | Start: 552 |
| Tm | 60.038 | 60.108 | End: 743 |
| Size | 20 | 20 | 192 |
| Primer 5 details |
|---|
| Sequence | GCTACATGACCGTCACACCA | GAACAACTCGGGGCATTTCG | Start: 27 |
| Tm | 60.038 | 59.832 | End: 241 |
| Size | 20 | 20 | 215 |
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