ssr_primer
Primer Details for TRINITY_DN73156_c2_g7_i1 |
|---|
| (A)21: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGACTTACTTGCCCCCGAAT | TTGTGCGCTGGTTATAGCCA | Start: 490 |
| Tm | 60.108 | 60.036 | End: 682 |
| Size | 20 | 20 | 193 |
| Primer 2 details |
|---|
| Sequence | TTTCCTCCTAACTCGCGACG | TTGTGCGCTGGTTATAGCCA | Start: 455 |
| Tm | 59.83 | 60.036 | End: 682 |
| Size | 20 | 20 | 228 |
| Primer 3 details |
|---|
| Sequence | TTGGAGGGGGTGCAAAAGTT | TGCTTGCCATGTTGCCAAAA | Start: 82 |
| Tm | 60.032 | 59.82 | End: 266 |
| Size | 20 | 20 | 185 |
| Primer 4 details |
|---|
| Sequence | TCTGAGCCTGGGCCATTTTT | ATTCGGGGGCAAGTAAGTCG | Start: 385 |
| Tm | 59.885 | 60.108 | End: 509 |
| Size | 20 | 20 | 125 |
| Primer 5 details |
|---|
| Sequence | TTGGAGGGGGTGCAAAAGTT | GTTTAATCCGACCAAGCGCG | Start: 82 |
| Tm | 60.032 | 60.248 | End: 345 |
| Size | 20 | 20 | 264 |
(AATAG)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGACTTACTTGCCCCCGAAT | TTGTGCGCTGGTTATAGCCA | Start: 490 |
| Tm | 60.108 | 60.036 | End: 682 |
| Size | 20 | 20 | 193 |
| Primer 2 details |
|---|
| Sequence | TTTCCTCCTAACTCGCGACG | TTGTGCGCTGGTTATAGCCA | Start: 455 |
| Tm | 59.83 | 60.036 | End: 682 |
| Size | 20 | 20 | 228 |
| Primer 3 details |
|---|
| Sequence | TTGGAGGGGGTGCAAAAGTT | TGCTTGCCATGTTGCCAAAA | Start: 82 |
| Tm | 60.032 | 59.82 | End: 266 |
| Size | 20 | 20 | 185 |
| Primer 4 details |
|---|
| Sequence | TCTGAGCCTGGGCCATTTTT | ATTCGGGGGCAAGTAAGTCG | Start: 385 |
| Tm | 59.885 | 60.108 | End: 509 |
| Size | 20 | 20 | 125 |
| Primer 5 details |
|---|
| Sequence | TTGGAGGGGGTGCAAAAGTT | GTTTAATCCGACCAAGCGCG | Start: 82 |
| Tm | 60.032 | 60.248 | End: 345 |
| Size | 20 | 20 | 264 |
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