ssr_primer
Primer Details for TRINITY_DN73149_c0_g1_i2 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GATCCACCTTCGGCACAGAA | GACCAGCCTGACCATGGAAA | Start: 1051 |
| Tm | 60.036 | 59.962 | End: 1230 |
| Size | 20 | 20 | 180 |
| Primer 2 details |
|---|
| Sequence | TGGAGTTCAGACGTGTGCTC | TGATTGTCACACTCACGCCA | Start: 43 |
| Tm | 59.968 | 59.894 | End: 272 |
| Size | 20 | 20 | 230 |
| Primer 3 details |
|---|
| Sequence | CTGGAGTTCAGACGTGTGCT | TGATTGTCACACTCACGCCA | Start: 42 |
| Tm | 59.967 | 59.894 | End: 272 |
| Size | 20 | 20 | 231 |
| Primer 4 details |
|---|
| Sequence | CCGGTGACTGGAGTTCAGAC | TGATTGTCACACTCACGCCA | Start: 35 |
| Tm | 60.038 | 59.894 | End: 272 |
| Size | 20 | 20 | 238 |
| Primer 5 details |
|---|
| Sequence | ATGCACTTGCGAGTACACGA | TGTGTGCTACCAGGGCAAAA | Start: 327 |
| Tm | 60.039 | 60.106 | End: 508 |
| Size | 20 | 20 | 182 |
(AAGA)4(AAG)(A)9: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GATCCACCTTCGGCACAGAA | GACCAGCCTGACCATGGAAA | Start: 1051 |
| Tm | 60.036 | 59.962 | End: 1230 |
| Size | 20 | 20 | 180 |
| Primer 2 details |
|---|
| Sequence | TGGAGTTCAGACGTGTGCTC | TGATTGTCACACTCACGCCA | Start: 43 |
| Tm | 59.968 | 59.894 | End: 272 |
| Size | 20 | 20 | 230 |
| Primer 3 details |
|---|
| Sequence | CTGGAGTTCAGACGTGTGCT | TGATTGTCACACTCACGCCA | Start: 42 |
| Tm | 59.967 | 59.894 | End: 272 |
| Size | 20 | 20 | 231 |
| Primer 4 details |
|---|
| Sequence | CCGGTGACTGGAGTTCAGAC | TGATTGTCACACTCACGCCA | Start: 35 |
| Tm | 60.038 | 59.894 | End: 272 |
| Size | 20 | 20 | 238 |
| Primer 5 details |
|---|
| Sequence | ATGCACTTGCGAGTACACGA | TGTGTGCTACCAGGGCAAAA | Start: 327 |
| Tm | 60.039 | 60.106 | End: 508 |
| Size | 20 | 20 | 182 |
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