ssr_primer
Primer Details for TRINITY_DN73142_c5_g1_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGATAAACAAAAGCCCGGAACA | AGTCTCGGTCTCGCAACAAG | Start: 2 |
| Tm | 59.441 | 60.039 | End: 132 |
| Size | 22 | 20 | 131 |
| Primer 2 details |
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| Sequence | GGATAAACAAAAGCCCGGAACA | ATCAGTCTCGGTCTCGCAAC | Start: 2 |
| Tm | 59.441 | 59.829 | End: 135 |
| Size | 22 | 20 | 134 |
| Primer 3 details |
|---|
| Sequence | AGGATAAACAAAAGCCCGGAAC | AGTCTCGGTCTCGCAACAAG | Start: 1 |
| Tm | 59.176 | 60.039 | End: 132 |
| Size | 22 | 20 | 132 |
| Primer 4 details |
|---|
| Sequence | GGATAAACAAAAGCCCGGAACA | CAGTCTCGGTCTCGCAACAA | Start: 2 |
| Tm | 59.441 | 60.319 | End: 133 |
| Size | 22 | 20 | 132 |
| Primer 5 details |
|---|
| Sequence | AGGATAAACAAAAGCCCGGA | AGTCTCGGTCTCGCAACAAG | Start: 1 |
| Tm | 57.109 | 60.039 | End: 132 |
| Size | 20 | 20 | 132 |
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