ssr_primer
Primer Details for TRINITY_DN73134_c2_g1_i1 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTAAGGGCGACGAGACTCT | AGCACAGTGTTGTTCCCCAA | Start: 848 |
| Tm | 60.035 | 60.034 | End: 1048 |
| Size | 20 | 20 | 201 |
| Primer 2 details |
|---|
| Sequence | AGTAAGGGCGACGAGACTCT | AAGCACAGTGTTGTTCCCCA | Start: 848 |
| Tm | 60.035 | 60.034 | End: 1049 |
| Size | 20 | 20 | 202 |
| Primer 3 details |
|---|
| Sequence | GAAACCATGCTCAGCCTCCT | AGAGTCTCGTCGCCCTTACT | Start: 643 |
| Tm | 60.035 | 60.035 | End: 867 |
| Size | 20 | 20 | 225 |
| Primer 4 details |
|---|
| Sequence | TTTGCGAAACCATGCTCAGC | AGAGTCTCGTCGCCCTTACT | Start: 638 |
| Tm | 60.039 | 60.035 | End: 867 |
| Size | 20 | 20 | 230 |
| Primer 5 details |
|---|
| Sequence | ACTTGTGCACATCGCTGAGA | ATTGATGGGGCACTGGTTGT | Start: 264 |
| Tm | 59.966 | 59.885 | End: 537 |
| Size | 20 | 20 | 274 |
(A)35: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTAAGGGCGACGAGACTCT | AGCACAGTGTTGTTCCCCAA | Start: 848 |
| Tm | 60.035 | 60.034 | End: 1048 |
| Size | 20 | 20 | 201 |
| Primer 2 details |
|---|
| Sequence | AGTAAGGGCGACGAGACTCT | AAGCACAGTGTTGTTCCCCA | Start: 848 |
| Tm | 60.035 | 60.034 | End: 1049 |
| Size | 20 | 20 | 202 |
| Primer 3 details |
|---|
| Sequence | GAAACCATGCTCAGCCTCCT | AGAGTCTCGTCGCCCTTACT | Start: 643 |
| Tm | 60.035 | 60.035 | End: 867 |
| Size | 20 | 20 | 225 |
| Primer 4 details |
|---|
| Sequence | TTTGCGAAACCATGCTCAGC | AGAGTCTCGTCGCCCTTACT | Start: 638 |
| Tm | 60.039 | 60.035 | End: 867 |
| Size | 20 | 20 | 230 |
| Primer 5 details |
|---|
| Sequence | ACTTGTGCACATCGCTGAGA | ATTGATGGGGCACTGGTTGT | Start: 264 |
| Tm | 59.966 | 59.885 | End: 537 |
| Size | 20 | 20 | 274 |
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