ssr_primer
Primer Details for TRINITY_DN73055_c5_g1_i1 |
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| (T)35: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGCTCCTTCCCCTTTTACG | TTGCTTCTTTCGCGCCTTTC | Start: 442 |
| Tm | 60.037 | 60.04 | End: 600 |
| Size | 20 | 20 | 159 |
| Primer 2 details |
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| Sequence | GAAACCATGCTACCCTCGCT | TTGCTTCTTTCGCGCCTTTC | Start: 402 |
| Tm | 60.108 | 60.04 | End: 600 |
| Size | 20 | 20 | 199 |
| Primer 3 details |
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| Sequence | GCCAAAGCTGGGTTCGAATC | CGTAAAAGGGGAAGGAGCGT | Start: 331 |
| Tm | 59.829 | 60.037 | End: 461 |
| Size | 20 | 20 | 131 |
| Primer 4 details |
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| Sequence | GCCAAAGCTGGGTTCGAATC | TTGCTTCTTTCGCGCCTTTC | Start: 331 |
| Tm | 59.829 | 60.04 | End: 600 |
| Size | 20 | 20 | 270 |
| Primer 5 details |
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| Sequence | GTCCGGCGTGACGAAAAATC | CGTAAAAGGGGAAGGAGCGT | Start: 351 |
| Tm | 60.179 | 60.037 | End: 461 |
| Size | 20 | 20 | 111 |
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