ssr_primer
Primer Details for TRINITY_DN73046_c3_g3_i2 |
|---|
| (G)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGTGGAATGTGACCGTCTG | TCCGCGACATGGTACCAAAA | Start: 1731 |
| Tm | 60.039 | 59.965 | End: 1925 |
| Size | 20 | 20 | 195 |
| Primer 2 details |
|---|
| Sequence | ACGATGTACACGACGAGACG | TCTTGTCCTGCTGCTGTCAG | Start: 674 |
| Tm | 59.906 | 59.966 | End: 832 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
|---|
| Sequence | ACGATGTACACGACGAGACG | AGTCTTGTCCTGCTGCTGTC | Start: 674 |
| Tm | 59.906 | 59.965 | End: 834 |
| Size | 20 | 20 | 161 |
| Primer 4 details |
|---|
| Sequence | CTGACAGCAGCAGGACAAGA | TGGTGACGCAAGATGTGACA | Start: 813 |
| Tm | 59.966 | 59.894 | End: 1060 |
| Size | 20 | 20 | 248 |
| Primer 5 details |
|---|
| Sequence | GACAGCAGCAGGACAAGACT | TGGTGACGCAAGATGTGACA | Start: 815 |
| Tm | 59.965 | 59.894 | End: 1060 |
| Size | 20 | 20 | 246 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGTGGAATGTGACCGTCTG | TCCGCGACATGGTACCAAAA | Start: 1731 |
| Tm | 60.039 | 59.965 | End: 1925 |
| Size | 20 | 20 | 195 |
| Primer 2 details |
|---|
| Sequence | ACGATGTACACGACGAGACG | TCTTGTCCTGCTGCTGTCAG | Start: 674 |
| Tm | 59.906 | 59.966 | End: 832 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
|---|
| Sequence | ACGATGTACACGACGAGACG | AGTCTTGTCCTGCTGCTGTC | Start: 674 |
| Tm | 59.906 | 59.965 | End: 834 |
| Size | 20 | 20 | 161 |
| Primer 4 details |
|---|
| Sequence | CTGACAGCAGCAGGACAAGA | TGGTGACGCAAGATGTGACA | Start: 813 |
| Tm | 59.966 | 59.894 | End: 1060 |
| Size | 20 | 20 | 248 |
| Primer 5 details |
|---|
| Sequence | GACAGCAGCAGGACAAGACT | TGGTGACGCAAGATGTGACA | Start: 815 |
| Tm | 59.965 | 59.894 | End: 1060 |
| Size | 20 | 20 | 246 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGTGGAATGTGACCGTCTG | TCCGCGACATGGTACCAAAA | Start: 1731 |
| Tm | 60.039 | 59.965 | End: 1925 |
| Size | 20 | 20 | 195 |
| Primer 2 details |
|---|
| Sequence | ACGATGTACACGACGAGACG | TCTTGTCCTGCTGCTGTCAG | Start: 674 |
| Tm | 59.906 | 59.966 | End: 832 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
|---|
| Sequence | ACGATGTACACGACGAGACG | AGTCTTGTCCTGCTGCTGTC | Start: 674 |
| Tm | 59.906 | 59.965 | End: 834 |
| Size | 20 | 20 | 161 |
| Primer 4 details |
|---|
| Sequence | CTGACAGCAGCAGGACAAGA | TGGTGACGCAAGATGTGACA | Start: 813 |
| Tm | 59.966 | 59.894 | End: 1060 |
| Size | 20 | 20 | 248 |
| Primer 5 details |
|---|
| Sequence | GACAGCAGCAGGACAAGACT | TGGTGACGCAAGATGTGACA | Start: 815 |
| Tm | 59.965 | 59.894 | End: 1060 |
| Size | 20 | 20 | 246 |
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