ssr_primer
Primer Details for TRINITY_DN73010_c8_g2_i1 |
|---|
| (C)20: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATAATAAGCCGCGGGGAGG | GCCCTTTATCACCCCAACGA | Start: 1163 |
| Tm | 59.964 | 60.035 | End: 1382 |
| Size | 20 | 20 | 220 |
| Primer 2 details |
|---|
| Sequence | TTGCCAGTGGAAATTTCGCG | AGAATAATAAGCGCCGCCGA | Start: 843 |
| Tm | 60.04 | 59.967 | End: 1109 |
| Size | 20 | 20 | 267 |
| Primer 3 details |
|---|
| Sequence | GGGCCCTGATAACGATCGAG | GCCCTTTATCACCCCAACGA | Start: 1131 |
| Tm | 60.038 | 60.035 | End: 1382 |
| Size | 20 | 20 | 252 |
| Primer 4 details |
|---|
| Sequence | AGTGAGAAGCTTTCCCGAGC | ACTGTGCGAGATAAACGGCA | Start: 522 |
| Tm | 60.037 | 60.038 | End: 690 |
| Size | 20 | 20 | 169 |
| Primer 5 details |
|---|
| Sequence | TGCCGTTTATCTCGCACAGT | CGCGAAATTTCCACTGGCAA | Start: 671 |
| Tm | 60.038 | 60.04 | End: 862 |
| Size | 20 | 20 | 192 |
(AAG)7aaaaatctaaactcagctagcatattttatgattaccacctgacgtgtttgtttcagatcacgaacttgcggcc(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATAATAAGCCGCGGGGAGG | GCCCTTTATCACCCCAACGA | Start: 1163 |
| Tm | 59.964 | 60.035 | End: 1382 |
| Size | 20 | 20 | 220 |
| Primer 2 details |
|---|
| Sequence | TTGCCAGTGGAAATTTCGCG | AGAATAATAAGCGCCGCCGA | Start: 843 |
| Tm | 60.04 | 59.967 | End: 1109 |
| Size | 20 | 20 | 267 |
| Primer 3 details |
|---|
| Sequence | GGGCCCTGATAACGATCGAG | GCCCTTTATCACCCCAACGA | Start: 1131 |
| Tm | 60.038 | 60.035 | End: 1382 |
| Size | 20 | 20 | 252 |
| Primer 4 details |
|---|
| Sequence | AGTGAGAAGCTTTCCCGAGC | ACTGTGCGAGATAAACGGCA | Start: 522 |
| Tm | 60.037 | 60.038 | End: 690 |
| Size | 20 | 20 | 169 |
| Primer 5 details |
|---|
| Sequence | TGCCGTTTATCTCGCACAGT | CGCGAAATTTCCACTGGCAA | Start: 671 |
| Tm | 60.038 | 60.04 | End: 862 |
| Size | 20 | 20 | 192 |
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