Primer Details for TRINITY_DN73003_c6_g1_i1 |
|---|
| (GAA)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAAGAAGAAGAGAAGAAGAACCA | TCGTCTTCTTCTTCTTTGTTCTTCT | Start: 88 |
| Tm | 57.261 | 58.426 | End: 228 |
| Size | 24 | 25 | 141 |
| Primer 2 details |
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| Sequence | AGAAGAAGAAGAGAAGAAGAACCA | TCGTCTTCTTCTTCTTTGTTCTTC | Start: 88 |
| Tm | 57.261 | 57.17 | End: 228 |
| Size | 24 | 24 | 141 |
| Primer 3 details |
|---|
| Sequence | AGAAGAAGAAGAGAAGAAGAACCA | CGTCTTCTTCTTCTTTGTTCTTCT | Start: 88 |
| Tm | 57.261 | 57.163 | End: 227 |
| Size | 24 | 24 | 140 |
| Primer 4 details |
|---|
| Sequence | AGAAGAAGAAGAGAAGAAGAACCAA | TCGTCTTCTTCTTCTTTGTTCTTCT | Start: 88 |
| Tm | 57.869 | 58.426 | End: 228 |
| Size | 25 | 25 | 141 |
| Primer 5 details |
|---|
| Sequence | AAGAAGAAGAAGAGAAGAAGAACCA | TCGTCTTCTTCTTCTTTGTTCTTCT | Start: 87 |
| Tm | 57.869 | 58.426 | End: 228 |
| Size | 25 | 25 | 142 |
