ssr_primer
Primer Details for TRINITY_DN72982_c1_g1_i2 |
|---|
| (AG)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAAAATGGGGGAGAGGGGG | CCGGCTTTTTCATCGCTACG | Start: 171 |
| Tm | 60.032 | 59.974 | End: 294 |
| Size | 20 | 20 | 124 |
| Primer 2 details |
|---|
| Sequence | AGCCAGAAATTCCCCATGGG | CGTTGGGGTTAGGGGTGTAC | Start: 474 |
| Tm | 60.032 | 60.036 | End: 704 |
| Size | 20 | 20 | 231 |
| Primer 3 details |
|---|
| Sequence | GTACACCCCTAACCCCAACG | TGCCATTTGCGAGAGGGAAT | Start: 685 |
| Tm | 60.036 | 60.034 | End: 842 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
|---|
| Sequence | CGTAGCGATGAAAAAGCCGG | CGGGATTGAGCCGTAGTTGT | Start: 275 |
| Tm | 59.974 | 60.108 | End: 386 |
| Size | 20 | 20 | 112 |
| Primer 5 details |
|---|
| Sequence | GAAAAATGGGGGAGAGGGGG | GTCCGTGTCCCACTCAAGTT | Start: 171 |
| Tm | 60.032 | 59.894 | End: 314 |
| Size | 20 | 20 | 144 |
(G)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAAAATGGGGGAGAGGGGG | CCGGCTTTTTCATCGCTACG | Start: 171 |
| Tm | 60.032 | 59.974 | End: 294 |
| Size | 20 | 20 | 124 |
| Primer 2 details |
|---|
| Sequence | AGCCAGAAATTCCCCATGGG | CGTTGGGGTTAGGGGTGTAC | Start: 474 |
| Tm | 60.032 | 60.036 | End: 704 |
| Size | 20 | 20 | 231 |
| Primer 3 details |
|---|
| Sequence | GTACACCCCTAACCCCAACG | TGCCATTTGCGAGAGGGAAT | Start: 685 |
| Tm | 60.036 | 60.034 | End: 842 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
|---|
| Sequence | CGTAGCGATGAAAAAGCCGG | CGGGATTGAGCCGTAGTTGT | Start: 275 |
| Tm | 59.974 | 60.108 | End: 386 |
| Size | 20 | 20 | 112 |
| Primer 5 details |
|---|
| Sequence | GAAAAATGGGGGAGAGGGGG | GTCCGTGTCCCACTCAAGTT | Start: 171 |
| Tm | 60.032 | 59.894 | End: 314 |
| Size | 20 | 20 | 144 |
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