Primer Details for TRINITY_DN72963_c10_g1_i1 |
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| (T)15atttttattga(T)24: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGAATCCTGATGACATCCTG | ACAACATACTCCTAATTTTTCTCCACA | Start: 104 |
| Tm | 59.559 | 59.058 | End: 215 |
| Size | 22 | 27 | 112 |
| Primer 2 details |
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| Sequence | GGGAATCCTGATGACATCCTGA | ACAACATACTCCTAATTTTTCTCCACA | Start: 105 |
| Tm | 59.292 | 59.058 | End: 215 |
| Size | 22 | 27 | 111 |
| Primer 3 details |
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| Sequence | TGGGAATCCTGATGACATCCT | ACAACATACTCCTAATTTTTCTCCACA | Start: 104 |
| Tm | 58.22 | 59.058 | End: 215 |
| Size | 21 | 27 | 112 |
| Primer 4 details |
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| Sequence | TGGGAATCCTGATGACATCCTG | CAACATACTCCTAATTTTTCTCCACA | Start: 104 |
| Tm | 59.559 | 57.664 | End: 214 |
| Size | 22 | 26 | 111 |
| Primer 5 details |
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| Sequence | TGGGAATCCTGATGACATCCTG | ACAACATACTCCTAATTTTTCTCCAC | Start: 104 |
| Tm | 59.559 | 57.663 | End: 215 |
| Size | 22 | 26 | 112 |
