Primer Details for TRINITY_DN72951_c12_g1_i1 |
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| (C)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGTTGGTCCAGAGTCCTT | TCGAATAATCAATATCGACAGGAAAAC | Start: 24 |
| Tm | 58.493 | 58.239 | End: 133 |
| Size | 20 | 27 | 110 |
| Primer 2 details |
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| Sequence | TTGTGTTGGTCCAGAGTCCT | TCGAATAATCAATATCGACAGGAAAAC | Start: 23 |
| Tm | 58.493 | 58.239 | End: 133 |
| Size | 20 | 27 | 111 |
| Primer 3 details |
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| Sequence | TGTGTTGGTCCAGAGTCCTT | CGAATAATCAATATCGACAGGAAAACT | Start: 24 |
| Tm | 58.493 | 58.235 | End: 132 |
| Size | 20 | 27 | 109 |
| Primer 4 details |
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| Sequence | TTGTGTTGGTCCAGAGTCCT | CGAATAATCAATATCGACAGGAAAACT | Start: 23 |
| Tm | 58.493 | 58.235 | End: 132 |
| Size | 20 | 27 | 110 |
| Primer 5 details |
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| Sequence | TGTGTTGGTCCAGAGTCCTT | TCGAATAATCAATATCGACAGGAAAA | Start: 24 |
| Tm | 58.493 | 57.119 | End: 133 |
| Size | 20 | 26 | 110 |
