Primer Details for TRINITY_DN72936_c2_g1_i1 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCTAGGCAAATAGTCAAATGTTCT | TGCCGCCCCTTAAATTTTGC | Start: 87 |
| Tm | 59.642 | 60.037 | End: 205 |
| Size | 25 | 20 | 119 |
| Primer 2 details | |||
| Sequence | AGTCAAATCAGGCATTTTGTCAA | TGCCGCCCCTTAAATTTTGC | Start: 62 |
| Tm | 57.466 | 60.037 | End: 205 |
| Size | 23 | 20 | 144 |
| Primer 3 details | |||
| Sequence | GCCTAGGCAAATAGTCAAATGTTC | TGCCGCCCCTTAAATTTTGC | Start: 87 |
| Tm | 58.417 | 60.037 | End: 205 |
| Size | 24 | 20 | 119 |
| Primer 4 details | |||
| Sequence | GCCTAGGCAAATAGTCAAATGTT | TGCCGCCCCTTAAATTTTGC | Start: 87 |
| Tm | 57.417 | 60.037 | End: 205 |
| Size | 23 | 20 | 119 |
| Primer 5 details | |||
| Sequence | AGTCAAATCAGGCATTTTGTCAA | GTCACCCCCTTAATCTGGCC | Start: 62 |
| Tm | 57.466 | 60.107 | End: 162 |
| Size | 23 | 20 | 101 |
