ssr_primer
Primer Details for TRINITY_DN72934_c2_g1_i3 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTTTCCATCCAAGCCCGAG | GACCCGTGGAGCATGTTGTA | Start: 2599 |
| Tm | 60.035 | 60.037 | End: 2821 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
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| Sequence | ACTTTCCATCCAAGCCCGAG | CTGGTCAGCGAAGTTTTGCC | Start: 2599 |
| Tm | 60.035 | 60.04 | End: 2733 |
| Size | 20 | 20 | 135 |
| Primer 3 details |
|---|
| Sequence | GGCAAAACTTCGCTGACCAG | GACCCGTGGAGCATGTTGTA | Start: 2714 |
| Tm | 60.04 | 60.037 | End: 2821 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
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| Sequence | TGGGGAGTACATACCTCGCA | TCAGCACTCTTGTCCACCAC | Start: 476 |
| Tm | 60.033 | 59.893 | End: 669 |
| Size | 20 | 20 | 194 |
| Primer 5 details |
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| Sequence | TGCGCAGGAGATGTAATGCA | TGCGAGGTATGTACTCCCCA | Start: 228 |
| Tm | 60.108 | 60.033 | End: 495 |
| Size | 20 | 20 | 268 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTTTCCATCCAAGCCCGAG | GACCCGTGGAGCATGTTGTA | Start: 2599 |
| Tm | 60.035 | 60.037 | End: 2821 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
|---|
| Sequence | ACTTTCCATCCAAGCCCGAG | CTGGTCAGCGAAGTTTTGCC | Start: 2599 |
| Tm | 60.035 | 60.04 | End: 2733 |
| Size | 20 | 20 | 135 |
| Primer 3 details |
|---|
| Sequence | GGCAAAACTTCGCTGACCAG | GACCCGTGGAGCATGTTGTA | Start: 2714 |
| Tm | 60.04 | 60.037 | End: 2821 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | TGGGGAGTACATACCTCGCA | TCAGCACTCTTGTCCACCAC | Start: 476 |
| Tm | 60.033 | 59.893 | End: 669 |
| Size | 20 | 20 | 194 |
| Primer 5 details |
|---|
| Sequence | TGCGCAGGAGATGTAATGCA | TGCGAGGTATGTACTCCCCA | Start: 228 |
| Tm | 60.108 | 60.033 | End: 495 |
| Size | 20 | 20 | 268 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTTTCCATCCAAGCCCGAG | GACCCGTGGAGCATGTTGTA | Start: 2599 |
| Tm | 60.035 | 60.037 | End: 2821 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
|---|
| Sequence | ACTTTCCATCCAAGCCCGAG | CTGGTCAGCGAAGTTTTGCC | Start: 2599 |
| Tm | 60.035 | 60.04 | End: 2733 |
| Size | 20 | 20 | 135 |
| Primer 3 details |
|---|
| Sequence | GGCAAAACTTCGCTGACCAG | GACCCGTGGAGCATGTTGTA | Start: 2714 |
| Tm | 60.04 | 60.037 | End: 2821 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | TGGGGAGTACATACCTCGCA | TCAGCACTCTTGTCCACCAC | Start: 476 |
| Tm | 60.033 | 59.893 | End: 669 |
| Size | 20 | 20 | 194 |
| Primer 5 details |
|---|
| Sequence | TGCGCAGGAGATGTAATGCA | TGCGAGGTATGTACTCCCCA | Start: 228 |
| Tm | 60.108 | 60.033 | End: 495 |
| Size | 20 | 20 | 268 |
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