ssr_primer
Primer Details for TRINITY_DN72913_c3_g1_i3 |
|---|
| (T)23: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTAGATGGCACCGAGCTTGG | CGTGATTCGGAGATCTGCGT | Start: 186 |
| Tm | 60.108 | 60.249 | End: 447 |
| Size | 20 | 20 | 262 |
| Primer 2 details |
|---|
| Sequence | TTAGATGGCACCGAGCTTGG | CGATTTCCTGCGTGATTCGG | Start: 186 |
| Tm | 60.108 | 59.698 | End: 457 |
| Size | 20 | 20 | 272 |
| Primer 3 details |
|---|
| Sequence | TTAGATGGCACCGAGCTTGG | TCGGAGATCTGCGTGATTGC | Start: 186 |
| Tm | 60.108 | 60.528 | End: 441 |
| Size | 20 | 20 | 256 |
| Primer 4 details |
|---|
| Sequence | TTAGATGGCACCGAGCTTGG | TGCGTGATTCGGAGATCTGC | Start: 186 |
| Tm | 60.108 | 60.528 | End: 449 |
| Size | 20 | 20 | 264 |
| Primer 5 details |
|---|
| Sequence | ACGCAGATCTCCGAATCACG | AGGGGAGGGGGATTAAAGGT | Start: 428 |
| Tm | 60.249 | 59.57 | End: 631 |
| Size | 20 | 20 | 204 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTAGATGGCACCGAGCTTGG | CGTGATTCGGAGATCTGCGT | Start: 186 |
| Tm | 60.108 | 60.249 | End: 447 |
| Size | 20 | 20 | 262 |
| Primer 2 details |
|---|
| Sequence | TTAGATGGCACCGAGCTTGG | CGATTTCCTGCGTGATTCGG | Start: 186 |
| Tm | 60.108 | 59.698 | End: 457 |
| Size | 20 | 20 | 272 |
| Primer 3 details |
|---|
| Sequence | TTAGATGGCACCGAGCTTGG | TCGGAGATCTGCGTGATTGC | Start: 186 |
| Tm | 60.108 | 60.528 | End: 441 |
| Size | 20 | 20 | 256 |
| Primer 4 details |
|---|
| Sequence | TTAGATGGCACCGAGCTTGG | TGCGTGATTCGGAGATCTGC | Start: 186 |
| Tm | 60.108 | 60.528 | End: 449 |
| Size | 20 | 20 | 264 |
| Primer 5 details |
|---|
| Sequence | ACGCAGATCTCCGAATCACG | AGGGGAGGGGGATTAAAGGT | Start: 428 |
| Tm | 60.249 | 59.57 | End: 631 |
| Size | 20 | 20 | 204 |
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