ssr_primer
Primer Details for TRINITY_DN72858_c4_g2_i1 |
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| (T)22ccccccaaaaaaaagggaacggcttttt(G)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAGGGAACGGCTTTTTGGG | CGCGCTCCATTTAGTCCCTA | Start: 61 |
| Tm | 59.244 | 59.612 | End: 253 |
| Size | 20 | 20 | 193 |
| Primer 2 details |
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| Sequence | AAAGGGAACGGCTTTTTGGG | AAGACGCTTTTCCTGGAGGC | Start: 61 |
| Tm | 59.244 | 60.607 | End: 299 |
| Size | 20 | 20 | 239 |
| Primer 3 details |
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| Sequence | AAGGGAACGGCTTTTTGGGG | CGCGCTCCATTTAGTCCCTA | Start: 62 |
| Tm | 61.119 | 59.612 | End: 253 |
| Size | 20 | 20 | 192 |
| Primer 4 details |
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| Sequence | AAAAGGGAACGGCTTTTTGGG | CGCGCTCCATTTAGTCCCTA | Start: 60 |
| Tm | 59.86 | 59.612 | End: 253 |
| Size | 21 | 20 | 194 |
| Primer 5 details |
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| Sequence | AAGGGAACGGCTTTTTGGGG | AAGACGCTTTTCCTGGAGGC | Start: 62 |
| Tm | 61.119 | 60.607 | End: 299 |
| Size | 20 | 20 | 238 |
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