ssr_primer
Primer Details for TRINITY_DN72855_c4_g1_i1 |
|---|
| (T)26: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCGAGTTGGATGTGGCCTT | CCAACTCGAGTGATGACCCC | Start: 115 |
| Tm | 60.036 | 60.108 | End: 322 |
| Size | 20 | 20 | 208 |
| Primer 2 details |
|---|
| Sequence | GGGGTCATCACTCGAGTTGG | AAGGCCACATCCAACTCGAG | Start: 105 |
| Tm | 60.108 | 60.036 | End: 332 |
| Size | 20 | 20 | 228 |
| Primer 3 details |
|---|
| Sequence | CTCGAGTTGGATGTGGCCTT | CGAGTGATGACCCCACAGAC | Start: 115 |
| Tm | 60.036 | 60.109 | End: 316 |
| Size | 20 | 20 | 202 |
| Primer 4 details |
|---|
| Sequence | GTCTGTGGGGTCATCACTCG | AAGGCCACATCCAACTCGAG | Start: 99 |
| Tm | 60.109 | 60.036 | End: 332 |
| Size | 20 | 20 | 234 |
| Primer 5 details |
|---|
| Sequence | TCACTCGAGTTGGATGTGGC | CCAACTCGAGTGATGACCCC | Start: 112 |
| Tm | 60.037 | 60.108 | End: 322 |
| Size | 20 | 20 | 211 |
(T)27: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCGAGTTGGATGTGGCCTT | CCAACTCGAGTGATGACCCC | Start: 115 |
| Tm | 60.036 | 60.108 | End: 322 |
| Size | 20 | 20 | 208 |
| Primer 2 details |
|---|
| Sequence | GGGGTCATCACTCGAGTTGG | AAGGCCACATCCAACTCGAG | Start: 105 |
| Tm | 60.108 | 60.036 | End: 332 |
| Size | 20 | 20 | 228 |
| Primer 3 details |
|---|
| Sequence | CTCGAGTTGGATGTGGCCTT | CGAGTGATGACCCCACAGAC | Start: 115 |
| Tm | 60.036 | 60.109 | End: 316 |
| Size | 20 | 20 | 202 |
| Primer 4 details |
|---|
| Sequence | GTCTGTGGGGTCATCACTCG | AAGGCCACATCCAACTCGAG | Start: 99 |
| Tm | 60.109 | 60.036 | End: 332 |
| Size | 20 | 20 | 234 |
| Primer 5 details |
|---|
| Sequence | TCACTCGAGTTGGATGTGGC | CCAACTCGAGTGATGACCCC | Start: 112 |
| Tm | 60.037 | 60.108 | End: 322 |
| Size | 20 | 20 | 211 |
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