ssr_primer
Primer Details for TRINITY_DN72847_c6_g1_i2 |
|---|
| (A)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTACCGGTAGTGAAGGGGG | AGGGGTTTTAGTGGGGGACT | Start: 722 |
| Tm | 60.033 | 60.104 | End: 838 |
| Size | 20 | 20 | 117 |
| Primer 2 details |
|---|
| Sequence | CACGCTCAAATACGCATGCA | GAGGACGCTTCGCTTGTTTC | Start: 17 |
| Tm | 59.903 | 59.836 | End: 155 |
| Size | 20 | 20 | 139 |
| Primer 3 details |
|---|
| Sequence | TCCGCGTTGCATATACACGA | TTGGGTCCTCGCATAAGAGC | Start: 241 |
| Tm | 59.899 | 59.822 | End: 368 |
| Size | 20 | 20 | 128 |
| Primer 4 details |
|---|
| Sequence | CACGCTCAAATACGCATGCA | CGTGTATATGCAACGCGGAC | Start: 17 |
| Tm | 59.903 | 59.771 | End: 259 |
| Size | 20 | 20 | 243 |
| Primer 5 details |
|---|
| Sequence | CACGCTCAAATACGCATGCA | GTGCTGGAGAGTCAAAACGC | Start: 17 |
| Tm | 59.903 | 59.764 | End: 288 |
| Size | 20 | 20 | 272 |
(G)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTACCGGTAGTGAAGGGGG | AGGGGTTTTAGTGGGGGACT | Start: 722 |
| Tm | 60.033 | 60.104 | End: 838 |
| Size | 20 | 20 | 117 |
| Primer 2 details |
|---|
| Sequence | CACGCTCAAATACGCATGCA | GAGGACGCTTCGCTTGTTTC | Start: 17 |
| Tm | 59.903 | 59.836 | End: 155 |
| Size | 20 | 20 | 139 |
| Primer 3 details |
|---|
| Sequence | TCCGCGTTGCATATACACGA | TTGGGTCCTCGCATAAGAGC | Start: 241 |
| Tm | 59.899 | 59.822 | End: 368 |
| Size | 20 | 20 | 128 |
| Primer 4 details |
|---|
| Sequence | CACGCTCAAATACGCATGCA | CGTGTATATGCAACGCGGAC | Start: 17 |
| Tm | 59.903 | 59.771 | End: 259 |
| Size | 20 | 20 | 243 |
| Primer 5 details |
|---|
| Sequence | CACGCTCAAATACGCATGCA | GTGCTGGAGAGTCAAAACGC | Start: 17 |
| Tm | 59.903 | 59.764 | End: 288 |
| Size | 20 | 20 | 272 |
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