ssr_primer
Primer Details for TRINITY_DN72836_c3_g5_i1 |
|---|
| (A)19: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCCACGCCTGTCTTCACTT | ACAAGACTCGGCAAGTGAGG | Start: 2626 |
| Tm | 59.966 | 59.966 | End: 2806 |
| Size | 20 | 20 | 181 |
| Primer 2 details |
|---|
| Sequence | AAGGCGCACGGAAATCCTAA | GCACTGCTTTACGTTTGCGA | Start: 758 |
| Tm | 60.036 | 60.042 | End: 943 |
| Size | 20 | 20 | 186 |
| Primer 3 details |
|---|
| Sequence | AAGGCGCACGGAAATCCTAA | TGGGGATCTCAGGTGCTGTA | Start: 758 |
| Tm | 60.036 | 59.957 | End: 965 |
| Size | 20 | 20 | 208 |
| Primer 4 details |
|---|
| Sequence | TAGCAGTTCCGGCATAGCTG | ATTGAATCGTGCCCAGTCGT | Start: 1689 |
| Tm | 59.895 | 60.036 | End: 1804 |
| Size | 20 | 20 | 116 |
| Primer 5 details |
|---|
| Sequence | ATTCGAGCCAAGAGTGAGCC | AAGTGAAGACAGGCGTGGAG | Start: 2487 |
| Tm | 60.108 | 59.966 | End: 2645 |
| Size | 20 | 20 | 159 |
(TATT)5(TATTTTTTTTGCTGTTGCAAAAAGAGCCGTTGGTCTGGCCGGAATTGAATTCTCACACATCTCATTCATCAACTAAAACAGTACTCAGGCCACAGAATCCGTTAGGTTTCGAGGGAAATTTTGAGCAAGTATCCGCATTGATGCAGAATAGATAGAAGGAGGAGAAAAAAAAGCTGTGTTACAATACTGTAAGGAGCATTGATGTATTAACTATGTATTTACGTAAAATATGTCTTTTTATCACCTAAGCTGCCAACAATAGCATAATTGCCAGAGGAAGCCCCCAACTCCTGATTTTATACCCGACCAAACATCTCATTACCTTTTTTGTCCTTAATCAGCAATCAGTGCTAATTCAAGGTTGAAATACATATGTAAATTTATTTATCTATGCCATCGATAAAAGCATGGTTGGTTGCCAGACGAGGCGCGACACTCCTAATTTTGTAAGTCACAAAACACCCCATTATTCCATCTTCCTTCCTTATTCGGCGCAATTAGAAAGAGGCATGGTTGATGGACCCAGCAGGAAACGTACTTTCATTGTTCACCTCTATACTACACATAATTGCACTTGTTTTGCACTATCATTGCACTTGTTTTGCTTTATCTGACAAAAGATGGTCTATTATCCTCATTACCTCCCTACTCTCACCACGGGCATATATTTCCTTATTAATGCTCCTAACATGGATTTAGTCATCCTCCTTCCATCCTTGTCATCCTTATTGAGACTAGTTTTTTGATCGTTTTTTGTTAATCGTGTTTTTTTCTCATGAAGAGTCTTTCTTTTAATTTTTTTTTTTTTTTTTTAATACCTGCCAACGTTTACGGCATCATTTTGGAAAAAAAAAAAAAAAATTGTCACAATTATTCATGATGGCGATTGAAACGGGCAGAAGACAAGGAGAGAGGGACTCCCAGTTATTTCTAAATTAGGAGGGTCCTACGATCACTGCAATAATATTGGTTTTTTTAACCAAGGATACAAGGGATCTACTTGCCATTTTTACCATTGATACCGAAAAGCACCATAATTCCAAATACAATATTAGCAGCAATAACAACCTATTAGCCGCTGGGTGCTTTTTTCCAATATAGGCATCAACAAGACTCATCTGAAAAATATAGAACATACGGGAGAAGAAGAAGATAACGTGACATGCAACCTGAATGATTCCAGGGGTGCAAAACCCTCCCAACTTCTTAACTCGCTTGGCTGCGTGAATGGAAGAATTATCAGATTTTTCCCAGATTTTGTGACCTTAGCGTTGTAGCACTAGAAAATTCAAGTTTTTTTGAGAGGATCAGGTTTTTTTTCGTCCGAGAAGGATCTGATCGTGAATTTGGTGAAAAATAGGAGCAGGTCAATTATTTTGAGACTGGAGAACTTGGAACTCTTCGCAGCTACTTCAGGTTAATCAGTGCGGTTGCTCAATTCAAAAATACATCATTATCCGCGGTGTCTGGCTACAGCGTAATTTTGGTCTTATAATGACCGTAGGATCCACCAGATTCAGAAACATCTAGGAGTCCCTCTCTTGAGCCCCGTCTTCTCCCCACTTTGTTCGCCATGATGAAAA)(T)9: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCCACGCCTGTCTTCACTT | ACAAGACTCGGCAAGTGAGG | Start: 2626 |
| Tm | 59.966 | 59.966 | End: 2806 |
| Size | 20 | 20 | 181 |
| Primer 2 details |
|---|
| Sequence | AAGGCGCACGGAAATCCTAA | GCACTGCTTTACGTTTGCGA | Start: 758 |
| Tm | 60.036 | 60.042 | End: 943 |
| Size | 20 | 20 | 186 |
| Primer 3 details |
|---|
| Sequence | AAGGCGCACGGAAATCCTAA | TGGGGATCTCAGGTGCTGTA | Start: 758 |
| Tm | 60.036 | 59.957 | End: 965 |
| Size | 20 | 20 | 208 |
| Primer 4 details |
|---|
| Sequence | TAGCAGTTCCGGCATAGCTG | ATTGAATCGTGCCCAGTCGT | Start: 1689 |
| Tm | 59.895 | 60.036 | End: 1804 |
| Size | 20 | 20 | 116 |
| Primer 5 details |
|---|
| Sequence | ATTCGAGCCAAGAGTGAGCC | AAGTGAAGACAGGCGTGGAG | Start: 2487 |
| Tm | 60.108 | 59.966 | End: 2645 |
| Size | 20 | 20 | 159 |
(T)18aatacctgccaacgtttacggcatcattttgg(A)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCCACGCCTGTCTTCACTT | ACAAGACTCGGCAAGTGAGG | Start: 2626 |
| Tm | 59.966 | 59.966 | End: 2806 |
| Size | 20 | 20 | 181 |
| Primer 2 details |
|---|
| Sequence | AAGGCGCACGGAAATCCTAA | GCACTGCTTTACGTTTGCGA | Start: 758 |
| Tm | 60.036 | 60.042 | End: 943 |
| Size | 20 | 20 | 186 |
| Primer 3 details |
|---|
| Sequence | AAGGCGCACGGAAATCCTAA | TGGGGATCTCAGGTGCTGTA | Start: 758 |
| Tm | 60.036 | 59.957 | End: 965 |
| Size | 20 | 20 | 208 |
| Primer 4 details |
|---|
| Sequence | TAGCAGTTCCGGCATAGCTG | ATTGAATCGTGCCCAGTCGT | Start: 1689 |
| Tm | 59.895 | 60.036 | End: 1804 |
| Size | 20 | 20 | 116 |
| Primer 5 details |
|---|
| Sequence | ATTCGAGCCAAGAGTGAGCC | AAGTGAAGACAGGCGTGGAG | Start: 2487 |
| Tm | 60.108 | 59.966 | End: 2645 |
| Size | 20 | 20 | 159 |
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