ssr_primer
Primer Details for TRINITY_DN72806_c2_g1_i1 |
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| (A)11tatccttaaatttggacattggagagaaa(G)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAACCGCTTTTCCTTGGTG | AAAAGTGCTGCCCAGACACT | Start: 12 |
| Tm | 59.968 | 60.107 | End: 136 |
| Size | 20 | 20 | 125 |
| Primer 2 details |
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| Sequence | TCCTTGGTGTTAACGCCTCC | AAAAGTGCTGCCCAGACACT | Start: 23 |
| Tm | 59.964 | 60.107 | End: 136 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
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| Sequence | CCAACCGCTTTTCCTTGGTG | TGCTGCCCAGACACTGATTT | Start: 12 |
| Tm | 59.968 | 59.889 | End: 131 |
| Size | 20 | 20 | 120 |
| Primer 4 details |
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| Sequence | TCCTTGGTGTTAACGCCTCC | TGCTGCCCAGACACTGATTT | Start: 23 |
| Tm | 59.964 | 59.889 | End: 131 |
| Size | 20 | 20 | 109 |
| Primer 5 details |
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| Sequence | GGGGTTCAGCTTCGGAAAGA | TGCGCCCTAAGGTTTCAGAA | Start: 412 |
| Tm | 59.964 | 59.601 | End: 557 |
| Size | 20 | 20 | 146 |
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