ssr_primer
Primer Details for TRINITY_DN72772_c4_g1_i5 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGCCGGAAGTAGTGGTTCC | ACAATGTCGCCAACGGAGAT | Start: 67 |
| Tm | 60.108 | 60.036 | End: 252 |
| Size | 20 | 20 | 186 |
| Primer 2 details |
|---|
| Sequence | ACCGGTGGCATTTTTACCCT | TGGCTTGCTGCTTCGTTAGA | Start: 476 |
| Tm | 59.886 | 59.965 | End: 751 |
| Size | 20 | 20 | 276 |
| Primer 3 details |
|---|
| Sequence | GAGCCGGAAGTAGTGGTTCC | CGATTTGCGAAGGTTGCACA | Start: 67 |
| Tm | 60.108 | 60.041 | End: 207 |
| Size | 20 | 20 | 141 |
| Primer 4 details |
|---|
| Sequence | ATCTCCGTTGGCGACATTGT | AGGGTAAAAATGCCACCGGT | Start: 233 |
| Tm | 60.036 | 59.886 | End: 495 |
| Size | 20 | 20 | 263 |
| Primer 5 details |
|---|
| Sequence | ATCTCCGTTGGCGACATTGT | ACCTCCATTGCGGTGTTTCA | Start: 233 |
| Tm | 60.036 | 60.179 | End: 435 |
| Size | 20 | 20 | 203 |
(A)38: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGCCGGAAGTAGTGGTTCC | ACAATGTCGCCAACGGAGAT | Start: 67 |
| Tm | 60.108 | 60.036 | End: 252 |
| Size | 20 | 20 | 186 |
| Primer 2 details |
|---|
| Sequence | ACCGGTGGCATTTTTACCCT | TGGCTTGCTGCTTCGTTAGA | Start: 476 |
| Tm | 59.886 | 59.965 | End: 751 |
| Size | 20 | 20 | 276 |
| Primer 3 details |
|---|
| Sequence | GAGCCGGAAGTAGTGGTTCC | CGATTTGCGAAGGTTGCACA | Start: 67 |
| Tm | 60.108 | 60.041 | End: 207 |
| Size | 20 | 20 | 141 |
| Primer 4 details |
|---|
| Sequence | ATCTCCGTTGGCGACATTGT | AGGGTAAAAATGCCACCGGT | Start: 233 |
| Tm | 60.036 | 59.886 | End: 495 |
| Size | 20 | 20 | 263 |
| Primer 5 details |
|---|
| Sequence | ATCTCCGTTGGCGACATTGT | ACCTCCATTGCGGTGTTTCA | Start: 233 |
| Tm | 60.036 | 60.179 | End: 435 |
| Size | 20 | 20 | 203 |
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