ssr_primer
Primer Details for TRINITY_DN72768_c3_g2_i3 |
|---|
| (A)10tcgccagtttgtgaacatcgaagagtatgcgcccggtagttaag(CTT)8: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCGCATGGATCCCTTTGAA | GACGAAGCTCAGTCGGTGAA | Start: 13 |
| Tm | 60.034 | 60.039 | End: 256 |
| Size | 20 | 20 | 244 |
| Primer 2 details |
|---|
| Sequence | TTCACCGACTGAGCTTCGTC | TACAAGTTGCAGCGACTGGT | Start: 237 |
| Tm | 60.039 | 59.894 | End: 441 |
| Size | 20 | 20 | 205 |
| Primer 3 details |
|---|
| Sequence | TGTAGGCTCCGGAGTACCAA | TACAAGTTGCAGCGACTGGT | Start: 315 |
| Tm | 59.96 | 59.894 | End: 441 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
|---|
| Sequence | GATACTGCTGGACGCTCTCC | AGAAGCTTAACTACCGGGCG | Start: 361 |
| Tm | 59.969 | 59.825 | End: 580 |
| Size | 20 | 20 | 220 |
| Primer 5 details |
|---|
| Sequence | GGATACTGCTGGACGCTCTC | AGAAGCTTAACTACCGGGCG | Start: 360 |
| Tm | 59.969 | 59.825 | End: 580 |
| Size | 20 | 20 | 221 |
(A)29: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCGCATGGATCCCTTTGAA | GACGAAGCTCAGTCGGTGAA | Start: 13 |
| Tm | 60.034 | 60.039 | End: 256 |
| Size | 20 | 20 | 244 |
| Primer 2 details |
|---|
| Sequence | TTCACCGACTGAGCTTCGTC | TACAAGTTGCAGCGACTGGT | Start: 237 |
| Tm | 60.039 | 59.894 | End: 441 |
| Size | 20 | 20 | 205 |
| Primer 3 details |
|---|
| Sequence | TGTAGGCTCCGGAGTACCAA | TACAAGTTGCAGCGACTGGT | Start: 315 |
| Tm | 59.96 | 59.894 | End: 441 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
|---|
| Sequence | GATACTGCTGGACGCTCTCC | AGAAGCTTAACTACCGGGCG | Start: 361 |
| Tm | 59.969 | 59.825 | End: 580 |
| Size | 20 | 20 | 220 |
| Primer 5 details |
|---|
| Sequence | GGATACTGCTGGACGCTCTC | AGAAGCTTAACTACCGGGCG | Start: 360 |
| Tm | 59.969 | 59.825 | End: 580 |
| Size | 20 | 20 | 221 |
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