ssr_primer
Primer Details for TRINITY_DN72763_c5_g8_i1 |
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| (A)13ttgtctacatgactggttagtcttgttcctgcatt(A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCCAATGACGGCTGACTGA | TGCCGTGCAGATTTTGTTGG | Start: 1313 |
| Tm | 60.037 | 59.968 | End: 1574 |
| Size | 20 | 20 | 262 |
| Primer 2 details |
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| Sequence | GTCCAATGACGGCTGACTGA | GTTCTGGGCTTTGCGTGAAG | Start: 1313 |
| Tm | 60.037 | 60.04 | End: 1511 |
| Size | 20 | 20 | 199 |
| Primer 3 details |
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| Sequence | CCAACAAAATCTGCACGGCA | GTGCGGCTAACACCATCAAC | Start: 1555 |
| Tm | 59.968 | 59.832 | End: 1668 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
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| Sequence | GCTTTGCACGTTCTAGCCAG | TCAGTCAGCCGTCATTGGAC | Start: 1131 |
| Tm | 59.833 | 60.037 | End: 1332 |
| Size | 20 | 20 | 202 |
| Primer 5 details |
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| Sequence | GACATGTTGCCATCACGCTC | CTTCGTCTGCTCGCTACACA | Start: 2080 |
| Tm | 59.902 | 60.11 | End: 2227 |
| Size | 20 | 20 | 148 |
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