ssr_primer
Primer Details for TRINITY_DN72675_c1_g2_i9 |
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| (A)23gaaaaacaaagagcaaac(A)10ggtaaatggaacgaccagacaaccaggc(G)19: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGTCTCTTGCGAGTATGGG | GGTTGCGTAGGGACAAAGGA | Start: 66 |
| Tm | 59.895 | 59.964 | End: 294 |
| Size | 20 | 20 | 229 |
| Primer 2 details |
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| Sequence | TGGAACGACCAGACAACCAG | CAACAGGCTTTGCATCACCC | Start: 715 |
| Tm | 59.894 | 60.038 | End: 908 |
| Size | 20 | 20 | 194 |
| Primer 3 details |
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| Sequence | GCCCGTTCCACCAATAGGAT | GGTTGCGTAGGGACAAAGGA | Start: 126 |
| Tm | 59.817 | 59.964 | End: 294 |
| Size | 20 | 20 | 169 |
| Primer 4 details |
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| Sequence | GCTCCCCTAGGGTAGAGAGG | CTGGTTGTCTGGTCGTTCCA | Start: 621 |
| Tm | 59.886 | 59.894 | End: 734 |
| Size | 20 | 20 | 114 |
| Primer 5 details |
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| Sequence | TGGAACGACCAGACAACCAG | ACAGGCTTTGCATCACCCTT | Start: 715 |
| Tm | 59.894 | 60.179 | End: 906 |
| Size | 20 | 20 | 192 |
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