ssr_primer
Primer Details for TRINITY_DN72553_c1_g3_i4 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCTGACACTGGCCAAAAA | CCACAAATGCGCACACATCA | Start: 442 |
| Tm | 60.033 | 60.039 | End: 601 |
| Size | 20 | 20 | 160 |
| Primer 2 details |
|---|
| Sequence | TTACAAACTCACCGCGCTCT | ATGACAAGCTCCCAGGCATC | Start: 2251 |
| Tm | 59.967 | 60.107 | End: 2404 |
| Size | 20 | 20 | 154 |
| Primer 3 details |
|---|
| Sequence | CAGAGACTGGTCATGGCTGG | TCTACGCTGATCTCGCCAAC | Start: 1812 |
| Tm | 60.108 | 59.899 | End: 1932 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
|---|
| Sequence | ACCCTGACACTGGCCAAAAA | AGACCGGCAGTGTGATATGC | Start: 442 |
| Tm | 60.033 | 60.179 | End: 551 |
| Size | 20 | 20 | 110 |
| Primer 5 details |
|---|
| Sequence | TTACAAACTCACCGCGCTCT | AATCCAGATCCTCGTGCAGC | Start: 2251 |
| Tm | 59.967 | 60.179 | End: 2494 |
| Size | 20 | 20 | 244 |
(A)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCTGACACTGGCCAAAAA | CCACAAATGCGCACACATCA | Start: 442 |
| Tm | 60.033 | 60.039 | End: 601 |
| Size | 20 | 20 | 160 |
| Primer 2 details |
|---|
| Sequence | TTACAAACTCACCGCGCTCT | ATGACAAGCTCCCAGGCATC | Start: 2251 |
| Tm | 59.967 | 60.107 | End: 2404 |
| Size | 20 | 20 | 154 |
| Primer 3 details |
|---|
| Sequence | CAGAGACTGGTCATGGCTGG | TCTACGCTGATCTCGCCAAC | Start: 1812 |
| Tm | 60.108 | 59.899 | End: 1932 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
|---|
| Sequence | ACCCTGACACTGGCCAAAAA | AGACCGGCAGTGTGATATGC | Start: 442 |
| Tm | 60.033 | 60.179 | End: 551 |
| Size | 20 | 20 | 110 |
| Primer 5 details |
|---|
| Sequence | TTACAAACTCACCGCGCTCT | AATCCAGATCCTCGTGCAGC | Start: 2251 |
| Tm | 59.967 | 60.179 | End: 2494 |
| Size | 20 | 20 | 244 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCTGACACTGGCCAAAAA | CCACAAATGCGCACACATCA | Start: 442 |
| Tm | 60.033 | 60.039 | End: 601 |
| Size | 20 | 20 | 160 |
| Primer 2 details |
|---|
| Sequence | TTACAAACTCACCGCGCTCT | ATGACAAGCTCCCAGGCATC | Start: 2251 |
| Tm | 59.967 | 60.107 | End: 2404 |
| Size | 20 | 20 | 154 |
| Primer 3 details |
|---|
| Sequence | CAGAGACTGGTCATGGCTGG | TCTACGCTGATCTCGCCAAC | Start: 1812 |
| Tm | 60.108 | 59.899 | End: 1932 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
|---|
| Sequence | ACCCTGACACTGGCCAAAAA | AGACCGGCAGTGTGATATGC | Start: 442 |
| Tm | 60.033 | 60.179 | End: 551 |
| Size | 20 | 20 | 110 |
| Primer 5 details |
|---|
| Sequence | TTACAAACTCACCGCGCTCT | AATCCAGATCCTCGTGCAGC | Start: 2251 |
| Tm | 59.967 | 60.179 | End: 2494 |
| Size | 20 | 20 | 244 |
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