ssr_primer
Primer Details for TRINITY_DN72510_c1_g3_i3 |
|---|
| (T)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAAAGAGGCCCAACGAGAA | AAATTGGCCGCATTTGGACC | Start: 170 |
| Tm | 59.964 | 60.036 | End: 321 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
|---|
| Sequence | GCCCTCGCACGTCTATAACA | GGAACGGAAAAGTGGCAACC | Start: 385 |
| Tm | 59.898 | 59.968 | End: 652 |
| Size | 20 | 20 | 268 |
| Primer 3 details |
|---|
| Sequence | GGAAAGAGGCCCAACGAGAA | TGTTATAGACGTGCGAGGGC | Start: 170 |
| Tm | 59.964 | 59.898 | End: 404 |
| Size | 20 | 20 | 235 |
| Primer 4 details |
|---|
| Sequence | GGTCCAAATGCGGCCAATTT | TGTTATAGACGTGCGAGGGC | Start: 302 |
| Tm | 60.036 | 59.898 | End: 404 |
| Size | 20 | 20 | 103 |
| Primer 5 details |
|---|
| Sequence | GTTAATGGCCTCCCGAACGA | GGAACGGAAAAGTGGCAACC | Start: 469 |
| Tm | 60.108 | 59.968 | End: 652 |
| Size | 20 | 20 | 184 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAAAGAGGCCCAACGAGAA | AAATTGGCCGCATTTGGACC | Start: 170 |
| Tm | 59.964 | 60.036 | End: 321 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
|---|
| Sequence | GCCCTCGCACGTCTATAACA | GGAACGGAAAAGTGGCAACC | Start: 385 |
| Tm | 59.898 | 59.968 | End: 652 |
| Size | 20 | 20 | 268 |
| Primer 3 details |
|---|
| Sequence | GGAAAGAGGCCCAACGAGAA | TGTTATAGACGTGCGAGGGC | Start: 170 |
| Tm | 59.964 | 59.898 | End: 404 |
| Size | 20 | 20 | 235 |
| Primer 4 details |
|---|
| Sequence | GGTCCAAATGCGGCCAATTT | TGTTATAGACGTGCGAGGGC | Start: 302 |
| Tm | 60.036 | 59.898 | End: 404 |
| Size | 20 | 20 | 103 |
| Primer 5 details |
|---|
| Sequence | GTTAATGGCCTCCCGAACGA | GGAACGGAAAAGTGGCAACC | Start: 469 |
| Tm | 60.108 | 59.968 | End: 652 |
| Size | 20 | 20 | 184 |
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