Primer Details for TRINITY_DN72501_c4_g1_i1 |
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| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCTTGAAGATTTATCAACTGGT | ACCTTGATTCACTAGCTTGCA | Start: 102 |
| Tm | 57.101 | 57.576 | End: 202 |
| Size | 24 | 21 | 101 |
| Primer 2 details |
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| Sequence | TGCTTGAAGATTTATCAACTGGTAACC | ACCTTGATTCACTAGCTTGCA | Start: 103 |
| Tm | 60.094 | 57.576 | End: 202 |
| Size | 27 | 21 | 100 |
| Primer 3 details |
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| Sequence | TTTGCTTGAAGATTTATCAACTGGT | ACCTTGATTCACTAGCTTGCA | Start: 101 |
| Tm | 57.711 | 57.576 | End: 202 |
| Size | 25 | 21 | 102 |
| Primer 4 details |
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| Sequence | TTGCTTGAAGATTTATCAACTGGT | ACCTTGATTCACTAGCTTGCAT | Start: 102 |
| Tm | 57.101 | 58.039 | End: 202 |
| Size | 24 | 22 | 101 |
| Primer 5 details |
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| Sequence | TGCTTGAAGATTTATCAACTGGTAACC | ACCTTGATTCACTAGCTTGCAT | Start: 103 |
| Tm | 60.094 | 58.039 | End: 202 |
| Size | 27 | 22 | 100 |
