ssr_primer
Primer Details for TRINITY_DN72492_c1_g1_i5 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTGGACTTGCAGTTGCTGG | CACTCTACGGCTGAGCTACG | Start: 1035 |
| Tm | 59.969 | 59.972 | End: 1159 |
| Size | 20 | 20 | 125 |
| Primer 2 details |
|---|
| Sequence | CGCACTCTGTTCTGAGTCGT | GAGGAACGTGCTTGGCAAAG | Start: 1327 |
| Tm | 60.04 | 60.04 | End: 1564 |
| Size | 20 | 20 | 238 |
| Primer 3 details |
|---|
| Sequence | CGTAGCTCAGCCGTAGAGTG | GCCGAGGCTAGTGCAAGTAT | Start: 1140 |
| Tm | 59.972 | 59.895 | End: 1381 |
| Size | 20 | 20 | 242 |
| Primer 4 details |
|---|
| Sequence | ATACTTGCACTAGCCTCGGC | GAGGAACGTGCTTGGCAAAG | Start: 1362 |
| Tm | 59.895 | 60.04 | End: 1564 |
| Size | 20 | 20 | 203 |
| Primer 5 details |
|---|
| Sequence | CGTAGCTCAGCCGTAGAGTG | TCCAACTGCAGACCAAACGA | Start: 1140 |
| Tm | 59.972 | 59.821 | End: 1362 |
| Size | 20 | 20 | 223 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTGGACTTGCAGTTGCTGG | CACTCTACGGCTGAGCTACG | Start: 1035 |
| Tm | 59.969 | 59.972 | End: 1159 |
| Size | 20 | 20 | 125 |
| Primer 2 details |
|---|
| Sequence | CGCACTCTGTTCTGAGTCGT | GAGGAACGTGCTTGGCAAAG | Start: 1327 |
| Tm | 60.04 | 60.04 | End: 1564 |
| Size | 20 | 20 | 238 |
| Primer 3 details |
|---|
| Sequence | CGTAGCTCAGCCGTAGAGTG | GCCGAGGCTAGTGCAAGTAT | Start: 1140 |
| Tm | 59.972 | 59.895 | End: 1381 |
| Size | 20 | 20 | 242 |
| Primer 4 details |
|---|
| Sequence | ATACTTGCACTAGCCTCGGC | GAGGAACGTGCTTGGCAAAG | Start: 1362 |
| Tm | 59.895 | 60.04 | End: 1564 |
| Size | 20 | 20 | 203 |
| Primer 5 details |
|---|
| Sequence | CGTAGCTCAGCCGTAGAGTG | TCCAACTGCAGACCAAACGA | Start: 1140 |
| Tm | 59.972 | 59.821 | End: 1362 |
| Size | 20 | 20 | 223 |
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