ssr_primer
Primer Details for TRINITY_DN72486_c5_g2_i1 |
|---|
| (A)23: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTTCCTCTTGCCCAAGCTC | TTCGAGAAATCGGCATGCCT | Start: 2853 |
| Tm | 59.963 | 60.108 | End: 3031 |
| Size | 20 | 20 | 179 |
| Primer 2 details |
|---|
| Sequence | ACTTCCTCTTGCCCAAGCTC | TGGCTTCGAGAAATCGGCAT | Start: 2853 |
| Tm | 59.963 | 60.108 | End: 3035 |
| Size | 20 | 20 | 183 |
| Primer 3 details |
|---|
| Sequence | ACTTCCTCTTGCCCAAGCTC | ATGGCTTCGAGAAATCGGCA | Start: 2853 |
| Tm | 59.963 | 60.108 | End: 3036 |
| Size | 20 | 20 | 184 |
| Primer 4 details |
|---|
| Sequence | CGAGGAGCTCCGGACATTTT | TGGCTGACATGGTTGCATCT | Start: 3245 |
| Tm | 60.108 | 59.961 | End: 3499 |
| Size | 20 | 20 | 255 |
| Primer 5 details |
|---|
| Sequence | TGCCCAAGCTCCAGCATTTA | TTCGAGAAATCGGCATGCCT | Start: 2862 |
| Tm | 59.961 | 60.108 | End: 3031 |
| Size | 20 | 20 | 170 |
(AAC)5aataataat(AAC)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTTCCTCTTGCCCAAGCTC | TTCGAGAAATCGGCATGCCT | Start: 2853 |
| Tm | 59.963 | 60.108 | End: 3031 |
| Size | 20 | 20 | 179 |
| Primer 2 details |
|---|
| Sequence | ACTTCCTCTTGCCCAAGCTC | TGGCTTCGAGAAATCGGCAT | Start: 2853 |
| Tm | 59.963 | 60.108 | End: 3035 |
| Size | 20 | 20 | 183 |
| Primer 3 details |
|---|
| Sequence | ACTTCCTCTTGCCCAAGCTC | ATGGCTTCGAGAAATCGGCA | Start: 2853 |
| Tm | 59.963 | 60.108 | End: 3036 |
| Size | 20 | 20 | 184 |
| Primer 4 details |
|---|
| Sequence | CGAGGAGCTCCGGACATTTT | TGGCTGACATGGTTGCATCT | Start: 3245 |
| Tm | 60.108 | 59.961 | End: 3499 |
| Size | 20 | 20 | 255 |
| Primer 5 details |
|---|
| Sequence | TGCCCAAGCTCCAGCATTTA | TTCGAGAAATCGGCATGCCT | Start: 2862 |
| Tm | 59.961 | 60.108 | End: 3031 |
| Size | 20 | 20 | 170 |
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