ssr_primer
Primer Details for TRINITY_DN72456_c7_g3_i1 |
|---|
| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGTTGAGGGAGGCTTCTGA | ACCCTCTCGATACGTGACCT | Start: 69 |
| Tm | 60.179 | 59.745 | End: 344 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
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| Sequence | AGGTCACGTATCGAGAGGGT | TGATTGGGCGGGCTATTGAA | Start: 325 |
| Tm | 59.745 | 59.743 | End: 594 |
| Size | 20 | 20 | 270 |
| Primer 3 details |
|---|
| Sequence | AGGTCACGTATCGAGAGGGT | GTGATTGGGCGGGCTATTGA | Start: 325 |
| Tm | 59.745 | 60.465 | End: 595 |
| Size | 20 | 20 | 271 |
| Primer 4 details |
|---|
| Sequence | AGGTTGAGGGAGGCTTCTGA | AGGACCCTCTCGATACGTGA | Start: 69 |
| Tm | 60.179 | 59.455 | End: 347 |
| Size | 20 | 20 | 279 |
| Primer 5 details |
|---|
| Sequence | TCACGTATCGAGAGGGTCCT | TGATTGGGCGGGCTATTGAA | Start: 328 |
| Tm | 59.455 | 59.743 | End: 594 |
| Size | 20 | 20 | 267 |
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