ssr_primer
Primer Details for TRINITY_DN72453_c3_g2_i4 |
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| (T)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTTCCCCTGAAAAGCCTCA | TACCGTATGGGATGCTTCGC | Start: 295 |
| Tm | 59.888 | 59.967 | End: 574 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
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| Sequence | CCACCCTTGTTAAGCCACCA | GGTGTCAGAGGAGGAGGCTA | Start: 770 |
| Tm | 60.179 | 60.033 | End: 967 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
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| Sequence | CATTCTCTGGCTCCCTCCAC | GGTGTCAGAGGAGGAGGCTA | Start: 835 |
| Tm | 59.82 | 60.033 | End: 967 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
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| Sequence | GAGCCACCCTTGTTAAGCCA | GGTGTCAGAGGAGGAGGCTA | Start: 767 |
| Tm | 60.251 | 60.033 | End: 967 |
| Size | 20 | 20 | 201 |
| Primer 5 details |
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| Sequence | TCTCAGAACTTCCCCACGGA | TTGGCACTTTCTTCCGCTCT | Start: 99 |
| Tm | 60.179 | 59.891 | End: 274 |
| Size | 20 | 20 | 176 |
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