ssr_primer
Primer Details for TRINITY_DN72449_c3_g1_i1 |
|---|
| (A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACATCATGGGACCCAGCAT | AAAAGCGCAGGAAAAGGCAG | Start: 965 |
| Tm | 60.106 | 59.967 | End: 1213 |
| Size | 20 | 20 | 249 |
| Primer 2 details |
|---|
| Sequence | AACTGGTCGTTTTGCATCGC | ATGCTGGGTCCCATGATGTG | Start: 863 |
| Tm | 60.04 | 60.106 | End: 984 |
| Size | 20 | 20 | 122 |
| Primer 3 details |
|---|
| Sequence | CTGCCTTTTCCTGCGCTTTT | CTCGGATCAGCCAATCTGCA | Start: 1194 |
| Tm | 59.967 | 60.179 | End: 1473 |
| Size | 20 | 20 | 280 |
| Primer 4 details |
|---|
| Sequence | CACGTTGTCGGATGGATGGA | TGACGGATGGAAAAAGGGCA | Start: 20 |
| Tm | 60.108 | 59.889 | End: 282 |
| Size | 20 | 20 | 263 |
| Primer 5 details |
|---|
| Sequence | TCCACGTTGTCGGATGGATG | TGACGGATGGAAAAAGGGCA | Start: 18 |
| Tm | 60.108 | 59.889 | End: 282 |
| Size | 20 | 20 | 265 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACATCATGGGACCCAGCAT | AAAAGCGCAGGAAAAGGCAG | Start: 965 |
| Tm | 60.106 | 59.967 | End: 1213 |
| Size | 20 | 20 | 249 |
| Primer 2 details |
|---|
| Sequence | AACTGGTCGTTTTGCATCGC | ATGCTGGGTCCCATGATGTG | Start: 863 |
| Tm | 60.04 | 60.106 | End: 984 |
| Size | 20 | 20 | 122 |
| Primer 3 details |
|---|
| Sequence | CTGCCTTTTCCTGCGCTTTT | CTCGGATCAGCCAATCTGCA | Start: 1194 |
| Tm | 59.967 | 60.179 | End: 1473 |
| Size | 20 | 20 | 280 |
| Primer 4 details |
|---|
| Sequence | CACGTTGTCGGATGGATGGA | TGACGGATGGAAAAAGGGCA | Start: 20 |
| Tm | 60.108 | 59.889 | End: 282 |
| Size | 20 | 20 | 263 |
| Primer 5 details |
|---|
| Sequence | TCCACGTTGTCGGATGGATG | TGACGGATGGAAAAAGGGCA | Start: 18 |
| Tm | 60.108 | 59.889 | End: 282 |
| Size | 20 | 20 | 265 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACATCATGGGACCCAGCAT | AAAAGCGCAGGAAAAGGCAG | Start: 965 |
| Tm | 60.106 | 59.967 | End: 1213 |
| Size | 20 | 20 | 249 |
| Primer 2 details |
|---|
| Sequence | AACTGGTCGTTTTGCATCGC | ATGCTGGGTCCCATGATGTG | Start: 863 |
| Tm | 60.04 | 60.106 | End: 984 |
| Size | 20 | 20 | 122 |
| Primer 3 details |
|---|
| Sequence | CTGCCTTTTCCTGCGCTTTT | CTCGGATCAGCCAATCTGCA | Start: 1194 |
| Tm | 59.967 | 60.179 | End: 1473 |
| Size | 20 | 20 | 280 |
| Primer 4 details |
|---|
| Sequence | CACGTTGTCGGATGGATGGA | TGACGGATGGAAAAAGGGCA | Start: 20 |
| Tm | 60.108 | 59.889 | End: 282 |
| Size | 20 | 20 | 263 |
| Primer 5 details |
|---|
| Sequence | TCCACGTTGTCGGATGGATG | TGACGGATGGAAAAAGGGCA | Start: 18 |
| Tm | 60.108 | 59.889 | End: 282 |
| Size | 20 | 20 | 265 |
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