ssr_primer
Primer Details for TRINITY_DN72425_c1_g1_i4 |
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| (T)13gtgtgtgtgttttaaagtatgtttctgcgattgtattttgttgtattcataatatgtgtgttccgtatggtgtttggacagtgtgggcagtataag(T)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCGCCCGGTCTGTAATATT | CGCGCTCTTCAACACTGATG | Start: 1685 |
| Tm | 60.25 | 59.906 | End: 1865 |
| Size | 20 | 20 | 181 |
| Primer 2 details |
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| Sequence | GCAAGGAACCATACCCTGCT | AAAAGGCCGCTCCACAATTG | Start: 300 |
| Tm | 60.034 | 59.682 | End: 539 |
| Size | 20 | 20 | 240 |
| Primer 3 details |
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| Sequence | ACTTGCTTGAACTCGCCCTT | TCTCGCAACTGTTTCCCACA | Start: 1145 |
| Tm | 60.179 | 59.821 | End: 1420 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
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| Sequence | AACTTGCTTGAACTCGCCCT | TCTCGCAACTGTTTCCCACA | Start: 1144 |
| Tm | 60.179 | 59.821 | End: 1420 |
| Size | 20 | 20 | 277 |
| Primer 5 details |
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| Sequence | AAACAGTTGCGAGACCGTCT | GAGCAAGCGTTCAACGTTGA | Start: 1407 |
| Tm | 59.896 | 59.697 | End: 1599 |
| Size | 20 | 20 | 193 |
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