ssr_primer
Primer Details for TRINITY_DN72397_c1_g1_i3 |
|---|
| (T)30: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCACTGTCCAACTGGAGACA | CGCAGGTGACTTTGACTGGA | Start: 979 |
| Tm | 59.893 | 60.25 | End: 1134 |
| Size | 20 | 20 | 156 |
| Primer 2 details |
|---|
| Sequence | AATGGCTGAGCACTGTCCAA | CGCAGGTGACTTTGACTGGA | Start: 970 |
| Tm | 59.889 | 60.25 | End: 1134 |
| Size | 20 | 20 | 165 |
| Primer 3 details |
|---|
| Sequence | GCACTGTCCAACTGGAGACA | AGGACAAGTTGGCTGTTCGG | Start: 979 |
| Tm | 59.893 | 60.535 | End: 1154 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
|---|
| Sequence | TGAGCACTGTCCAACTGGAG | CGCAGGTGACTTTGACTGGA | Start: 976 |
| Tm | 59.606 | 60.25 | End: 1134 |
| Size | 20 | 20 | 159 |
| Primer 5 details |
|---|
| Sequence | CTGAGCACTGTCCAACTGGA | CGCAGGTGACTTTGACTGGA | Start: 975 |
| Tm | 59.606 | 60.25 | End: 1134 |
| Size | 20 | 20 | 160 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCACTGTCCAACTGGAGACA | CGCAGGTGACTTTGACTGGA | Start: 979 |
| Tm | 59.893 | 60.25 | End: 1134 |
| Size | 20 | 20 | 156 |
| Primer 2 details |
|---|
| Sequence | AATGGCTGAGCACTGTCCAA | CGCAGGTGACTTTGACTGGA | Start: 970 |
| Tm | 59.889 | 60.25 | End: 1134 |
| Size | 20 | 20 | 165 |
| Primer 3 details |
|---|
| Sequence | GCACTGTCCAACTGGAGACA | AGGACAAGTTGGCTGTTCGG | Start: 979 |
| Tm | 59.893 | 60.535 | End: 1154 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
|---|
| Sequence | TGAGCACTGTCCAACTGGAG | CGCAGGTGACTTTGACTGGA | Start: 976 |
| Tm | 59.606 | 60.25 | End: 1134 |
| Size | 20 | 20 | 159 |
| Primer 5 details |
|---|
| Sequence | CTGAGCACTGTCCAACTGGA | CGCAGGTGACTTTGACTGGA | Start: 975 |
| Tm | 59.606 | 60.25 | End: 1134 |
| Size | 20 | 20 | 160 |
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