ssr_primer
Primer Details for TRINITY_DN72395_c4_g2_i1 |
|---|
| (T)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGCGCCTTAAAATCCCCAT | AACGTCGCAATCGGAGGAAT | Start: 77 |
| Tm | 60.033 | 60.108 | End: 329 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
|---|
| Sequence | ATTCCTCCGATTGCGACGTT | GTAGGTAGTCGCGATTCCCG | Start: 310 |
| Tm | 60.108 | 60.041 | End: 584 |
| Size | 20 | 20 | 275 |
| Primer 3 details |
|---|
| Sequence | ATTCCTCCGATTGCGACGTT | CGTAGGTAGTCGCGATTCCC | Start: 310 |
| Tm | 60.108 | 60.041 | End: 585 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
|---|
| Sequence | TGGCGCCTTAAAATCCCCAT | CTCTTCGTCGTTTTCTGCGC | Start: 77 |
| Tm | 60.033 | 60.179 | End: 287 |
| Size | 20 | 20 | 211 |
| Primer 5 details |
|---|
| Sequence | ATGAGTTAAAGCGCCCCGAA | CTCTTCGTCGTTTTCTGCGC | Start: 11 |
| Tm | 60.036 | 60.179 | End: 287 |
| Size | 20 | 20 | 277 |
(A)12(ATAAATAAATAAATAAATAAGTAAAGGGGAATTGTTCTCCACTCTCCAACTCATTCTTTGAACCAGGAAAAGTCTAAGTCTTTGGGTAGGGAGAGGAGTTGACAAACTTTCCCGCTGTCTCTCCGGAAAAGAGAAACGAT)(AAAT)4: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGCGCCTTAAAATCCCCAT | AACGTCGCAATCGGAGGAAT | Start: 77 |
| Tm | 60.033 | 60.108 | End: 329 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
|---|
| Sequence | ATTCCTCCGATTGCGACGTT | GTAGGTAGTCGCGATTCCCG | Start: 310 |
| Tm | 60.108 | 60.041 | End: 584 |
| Size | 20 | 20 | 275 |
| Primer 3 details |
|---|
| Sequence | ATTCCTCCGATTGCGACGTT | CGTAGGTAGTCGCGATTCCC | Start: 310 |
| Tm | 60.108 | 60.041 | End: 585 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
|---|
| Sequence | TGGCGCCTTAAAATCCCCAT | CTCTTCGTCGTTTTCTGCGC | Start: 77 |
| Tm | 60.033 | 60.179 | End: 287 |
| Size | 20 | 20 | 211 |
| Primer 5 details |
|---|
| Sequence | ATGAGTTAAAGCGCCCCGAA | CTCTTCGTCGTTTTCTGCGC | Start: 11 |
| Tm | 60.036 | 60.179 | End: 287 |
| Size | 20 | 20 | 277 |
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