ssr_primer
Primer Details for TRINITY_DN72377_c0_g1_i3 |
|---|
| (A)21: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCATTTGTTGGGGGCACA | ATCCGTGGTTCTGCAGGTTT | Start: 490 |
| Tm | 60.106 | 59.89 | End: 691 |
| Size | 20 | 20 | 202 |
| Primer 2 details |
|---|
| Sequence | GAGCTGAGATGGGTCAGTGG | CGCAATGACGTCACAAAGCA | Start: 853 |
| Tm | 59.822 | 60.041 | End: 1014 |
| Size | 20 | 20 | 162 |
| Primer 3 details |
|---|
| Sequence | GAGCTGAGATGGGTCAGTGG | TGCGCAATGACGTCACAAAG | Start: 853 |
| Tm | 59.822 | 60.041 | End: 1016 |
| Size | 20 | 20 | 164 |
| Primer 4 details |
|---|
| Sequence | GAGCTGAGATGGGTCAGTGG | CTGCGCAATGACGTCACAAA | Start: 853 |
| Tm | 59.822 | 60.041 | End: 1017 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | TTTGTGACGTCATTGCGCAG | ATGGGTCAGTGGCAGTTGTT | Start: 998 |
| Tm | 60.041 | 59.814 | End: 1176 |
| Size | 20 | 20 | 179 |
(C)24: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCATTTGTTGGGGGCACA | ATCCGTGGTTCTGCAGGTTT | Start: 490 |
| Tm | 60.106 | 59.89 | End: 691 |
| Size | 20 | 20 | 202 |
| Primer 2 details |
|---|
| Sequence | GAGCTGAGATGGGTCAGTGG | CGCAATGACGTCACAAAGCA | Start: 853 |
| Tm | 59.822 | 60.041 | End: 1014 |
| Size | 20 | 20 | 162 |
| Primer 3 details |
|---|
| Sequence | GAGCTGAGATGGGTCAGTGG | TGCGCAATGACGTCACAAAG | Start: 853 |
| Tm | 59.822 | 60.041 | End: 1016 |
| Size | 20 | 20 | 164 |
| Primer 4 details |
|---|
| Sequence | GAGCTGAGATGGGTCAGTGG | CTGCGCAATGACGTCACAAA | Start: 853 |
| Tm | 59.822 | 60.041 | End: 1017 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | TTTGTGACGTCATTGCGCAG | ATGGGTCAGTGGCAGTTGTT | Start: 998 |
| Tm | 60.041 | 59.814 | End: 1176 |
| Size | 20 | 20 | 179 |
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